FLD Antibody

Datasheet

Code	CSB-PA863250XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FLD Polyclonal antibody

Uniprot No.

Q9CAE3

Target Names

FLD

Alternative Names

FLD antibody; SOF1 antibody; At3g10390 antibody; F13M14.34 antibody; F14P13.1Protein FLOWERING LOCUS D antibody; EC 1.-.-.- antibody; Protein SUPPRESSOR OF OVEREXPRESSED FCA 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana FLD protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Probable histone demethylase that promotes flowering independently of the photoperiod and vernalization pathways by repressing FLOWERING LOCUS C (FLC), a floral repressor that blocks the transition from vegetative to reproductive development. Probably mediates histone H3 'Lys-4' demethylation at FLC locus. Seems to act in partial redundancy with LDL1 and LDL2 to repress FLC expression. Required for histone H4 deacetylation of FLC locus. May be a component of the histone deacetylase complex. Forms a histone deacetylase complex with HDA5, HDA6 and MSI4/FVE that represses FLC gene expression to control flowering time.

Gene References into Functions

Thus, plants most likely direct their KDM1 chromatin-modifying enzymes to methylated histones of the target chromatin by a mechanism different from that employed by the mammalian cells. PMID: 26826387
HDA6 and FLD could act together in a protein complex. Increased levels of histone H3 acetylation and H3K4 trimethylation, indicating functional interplay between histone deacetylase and demethylase through HDA6 and FLD interaction in flowering control. PMID: 21398257
Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. PMID: 24499796
Data indicate that conformational changes of BRASSINAZOLE-RESISTANT1 (BZR1), mediated by cyclophilin (CYP20-2), may directly regulate the expression of FLOWERING LOCUS D (FLD) to influence flowering. PMID: 23897924
FLD's function in systemic acquired resistance (SAR) is independent of FLC. PMID: 23745676
There is a close association of FCA and FLD in mediating H3K4 demethylation and thus transcriptional silencing of FLC. PMID: 17996704
Data show that by repressing FLD activity through sumoylation, SIZ1 promoted FLC expression. PMID: 18069938

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Protein Families

Flavin monoamine oxidase family

Database Links

KEGG: ath:AT3G10390

STRING: 3702.AT3G10390.1

UniGene: At.39938

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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FLD Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background