Recombinant Arabidopsis thaliana Lysine-specific histone demethylase 1 homolog 3 (FLD), partial

Code CSB-YP863250DOA
MSDS
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Source Yeast
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Code CSB-EP863250DOA
MSDS
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Source E.coli
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Code CSB-EP863250DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863250DOA
MSDS
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Source Baculovirus
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Code CSB-MP863250DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FLD
Uniprot No.
Alternative Names
FLD; SOF1; At3g10390; F13M14.34; F14P13.1Protein FLOWERING LOCUS D; EC 1.-.-.-; Protein SUPPRESSOR OF OVEREXPRESSED FCA 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable histone demethylase that promotes flowering independently of the photoperiod and vernalization pathways by repressing FLOWERING LOCUS C (FLC), a floral repressor that blocks the transition from vegetative to reproductive development. Probably mediates histone H3 'Lys-4' demethylation at FLC locus. Seems to act in partial redundancy with LDL1 and LDL2 to repress FLC expression. Required for histone H4 deacetylation of FLC locus. May be a component of the histone deacetylase complex. Forms a histone deacetylase complex with HDA5, HDA6 and MSI4/FVE that represses FLC gene expression to control flowering time.
Gene References into Functions
  1. Thus, plants most likely direct their KDM1 chromatin-modifying enzymes to methylated histones of the target chromatin by a mechanism different from that employed by the mammalian cells. PMID: 26826387
  2. HDA6 and FLD could act together in a protein complex. Increased levels of histone H3 acetylation and H3K4 trimethylation, indicating functional interplay between histone deacetylase and demethylase through HDA6 and FLD interaction in flowering control. PMID: 21398257
  3. Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. PMID: 24499796
  4. Data indicate that conformational changes of BRASSINAZOLE-RESISTANT1 (BZR1), mediated by cyclophilin (CYP20-2), may directly regulate the expression of FLOWERING LOCUS D (FLD) to influence flowering. PMID: 23897924
  5. FLD's function in systemic acquired resistance (SAR) is independent of FLC. PMID: 23745676
  6. There is a close association of FCA and FLD in mediating H3K4 demethylation and thus transcriptional silencing of FLC. PMID: 17996704
  7. Data show that by repressing FLD activity through sumoylation, SIZ1 promoted FLC expression. PMID: 18069938

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Protein Families
Flavin monoamine oxidase family
Database Links

KEGG: ath:AT3G10390

STRING: 3702.AT3G10390.1

UniGene: At.39938

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