FLO1 Antibody

Code CSB-PA330196XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FLO1 Polyclonal antibody
Uniprot No.
Target Names
FLO1
Alternative Names
FLO1 antibody; FLO2 antibody; FLO4 antibody; FLO8 antibody; YAR050WFlocculation protein FLO1 antibody; Flocculin-1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FLO1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion.
Gene References into Functions
  1. Sas3 and Ada2-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene. PMID: 28115623
  2. expression of FLO5 gene was higher than that of FLO1 gene PMID: 25218464
  3. Deletion of tandem repeats B, C and D adjacent to the 3'-terminal of FLO1 increases the conformation of flocculin in response to changes of pH and temperature. PMID: 24697100
  4. Expression of Lg-FLO1, which is required for flocculation of bottom-fermenting yeast, was also found to be induced by starvation for nitrogen. PMID: 23065862
  5. Repeated DNA sequence of FLO1 is important for the control of flocculation and genetic stability. PMID: 23383507
  6. N-terminal domain of Flo1p contains two mannose carbohydrate binding sites with different affinities PMID: 21076009
  7. The results indicate that FLONL and FLONS are both derived forms of the FLO1 gene; genetic variability triggered by tandem repeats in FLO1 gene is believed to be responsible for the differential phenotypic properties of the yeast strains YTS-S and YTS-L. PMID: 17662052
  8. FLONS, conferring NewFlo-type flocculation ability in yeast was cloned and The 3,396-bp ORF encoded a peptide of 1,132 amino acids with high identity to Flo1 protein PMID: 18057865
  9. FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast PMID: 19013280
  10. The authors describe the discovery of an extracellular matrix produced by FLO1-expressing Saccharomyces cerevisiae cells. PMID: 19207290

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor. Secreted, cell wall. Note=Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as covalently-linked GPI-modified cell wall protein (GPI-CWP) in the outer cell wall layer.
Protein Families
Flocculin family
Database Links

KEGG: sce:YAR050W

STRING: 4932.YAR050W

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