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Recombinant Saccharomyces cerevisiae Flocculation protein FLO1 (FLO1), partial

Recombinant Saccharomyces cerevisiae Flocculation protein FLO1 (FLO1), partial

Code	CSB-YP330196SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP330196SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP330196SVG-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP330196SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP330196SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

FLO1

Uniprot No.

P32768

Alternative Names

FLO1; FLO2; FLO4; FLO8; YAR050WFlocculation protein FLO1; Flocculin-1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Also involved in cell-substrate adhesion.

Gene References into Functions

Sas3 and Ada2-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene. PMID: 28115623
expression of FLO5 gene was higher than that of FLO1 gene PMID: 25218464
Deletion of tandem repeats B, C and D adjacent to the 3'-terminal of FLO1 increases the conformation of flocculin in response to changes of pH and temperature. PMID: 24697100
Expression of Lg-FLO1, which is required for flocculation of bottom-fermenting yeast, was also found to be induced by starvation for nitrogen. PMID: 23065862
Repeated DNA sequence of FLO1 is important for the control of flocculation and genetic stability. PMID: 23383507
N-terminal domain of Flo1p contains two mannose carbohydrate binding sites with different affinities PMID: 21076009
The results indicate that FLONL and FLONS are both derived forms of the FLO1 gene; genetic variability triggered by tandem repeats in FLO1 gene is believed to be responsible for the differential phenotypic properties of the yeast strains YTS-S and YTS-L. PMID: 17662052
FLONS, conferring NewFlo-type flocculation ability in yeast was cloned and The 3,396-bp ORF encoded a peptide of 1,132 amino acids with high identity to Flo1 protein PMID: 18057865
FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast PMID: 19013280
The authors describe the discovery of an extracellular matrix produced by FLO1-expressing Saccharomyces cerevisiae cells. PMID: 19207290

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Secreted, cell wall. Note=Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as covalently-linked GPI-modified cell wall protein (GPI-CWP) in the outer cell wall layer.

Protein Families

Flocculin family

Database Links

KEGG: sce:YAR050W

STRING: 4932.YAR050W

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