fj Antibody

Code CSB-PA347491XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) fj Polyclonal antibody
Uniprot No.
Target Names
fj
Alternative Names
fj antibody; CG10917Extracellular serine/threonine protein kinase four-jointed antibody; EC 2.7.11.1) [Cleaved into: Protein four-jointed antibody; secreted isoform] antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) fj protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Golgi serine/threonine protein kinase required for intermediate growth in the proximal-distal axis. Phosphorylates specific residues within extracellular cadherin domains of Fat (ft) and Dachsous (ds) as they transit through the Golgi. Acts in ommatidial polarity determination as a secondary signal downstream of Notch, JAK/STAT and wingless. Also necessary for the initiation, up-regulation or maintenance of Notch ligand, Serrate (Ser) expression in legs, thereby participating in a feedback loop with N signaling. Sufficient for joint formation and growth in the leg.
Gene References into Functions
  1. Four-jointed acts both on Fat to promote its binding to Dachsous and on Dachsous to inhibit its binding to Fat, with a bias towards a stronger effect on Fat PMID: 25707557
  2. modulates fat:daschsous binding and polarization activity via phosphorylation site PMID: 20434335
  3. effects on wing size and planar polarity by phosphorylating fat (ft) and dachsous (ds) activity PMID: 20434337
  4. The gradient of Fj and Ds expression provide partially redundant positional information essential for specifying the polarization. axis PMID: 15548581
  5. Wnt oncogene analog 4 antagonizes the polarizing effect of Fj PMID: 15986451

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Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein. Note=Golgi apparatus, in the wing pouch.; [Protein four-jointed, secreted isoform]: Secreted.
Protein Families
FJX1/FJ family
Tissue Specificity
In the eye disk, expressed in a gradient ahead of the morphogenetic furrow, high at the equator and low at the poles of the eye. In the leg disk, expressed in concentric rings, possibly corresponding to segmental boundaries. In the wing disk, expression i
Database Links

KEGG: dme:Dmel_CG10917

STRING: 7227.FBpp0085933

UniGene: Dm.4763

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