Recombinant Drosophila melanogaster Protein four-jointed (fj), partial

Code CSB-YP347491DLU
MSDS
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Source Yeast
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Code CSB-EP347491DLU
MSDS
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Source E.coli
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Code CSB-EP347491DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP347491DLU
MSDS
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Source Baculovirus
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Code CSB-MP347491DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
fj
Uniprot No.
Alternative Names
fj; CG10917Extracellular serine/threonine protein kinase four-jointed; EC 2.7.11.1) [Cleaved into: Protein four-jointed; secreted isoform]
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Golgi serine/threonine protein kinase required for intermediate growth in the proximal-distal axis. Phosphorylates specific residues within extracellular cadherin domains of Fat (ft) and Dachsous (ds) as they transit through the Golgi. Acts in ommatidial polarity determination as a secondary signal downstream of Notch, JAK/STAT and wingless. Also necessary for the initiation, up-regulation or maintenance of Notch ligand, Serrate (Ser) expression in legs, thereby participating in a feedback loop with N signaling. Sufficient for joint formation and growth in the leg.
Gene References into Functions
  1. Four-jointed acts both on Fat to promote its binding to Dachsous and on Dachsous to inhibit its binding to Fat, with a bias towards a stronger effect on Fat PMID: 25707557
  2. modulates fat:daschsous binding and polarization activity via phosphorylation site PMID: 20434335
  3. effects on wing size and planar polarity by phosphorylating fat (ft) and dachsous (ds) activity PMID: 20434337
  4. The gradient of Fj and Ds expression provide partially redundant positional information essential for specifying the polarization. axis PMID: 15548581
  5. Wnt oncogene analog 4 antagonizes the polarizing effect of Fj PMID: 15986451

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Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein. Note=Golgi apparatus, in the wing pouch.; [Protein four-jointed, secreted isoform]: Secreted.
Protein Families
FJX1/FJ family
Tissue Specificity
In the eye disk, expressed in a gradient ahead of the morphogenetic furrow, high at the equator and low at the poles of the eye. In the leg disk, expressed in concentric rings, possibly corresponding to segmental boundaries. In the wing disk, expression i
Database Links

KEGG: dme:Dmel_CG10917

STRING: 7227.FBpp0085933

UniGene: Dm.4763

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