FRI Antibody

Code CSB-PA727843XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FRI Polyclonal antibody
Uniprot No.
Target Names
FRI
Alternative Names
FRI antibody; At4g00650 antibody; F6N23.25Inactive protein FRIGIDA antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FRI protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Gene References into Functions
  1. Data indicate that FRIGIDA protein (FRI) as an activator of EARLY FLOWERING IN SHORT DAYS protein EFS (EFS) and that EFS was a histone H3 Lys36-specific methyltransferase. PMID: 28584923
  2. natural variation involving deletion alleles modulate temperature-sensitive flowering responses PMID: 26662639
  3. Structural Analysis of FRIGIDA Flowering-Time Regulator PMID: 26633860
  4. Proteasome-mediated FRI degradation regulates flowering during vernalization in Arabidopsis. PMID: 25538183
  5. Analysis of flowering time data from various Arabidopsis natural accessions indicated that the FRI-Ler allele retains some functionality. PMID: 25207670
  6. The expression of FRI gene in Arabidopsis thaliana plants in extreme conditions in northern margins of species range. PMID: 25474881
  7. The spatial patterns of FRI, FLC, and PHYC polymorphisms are significantly associated with winter temperatures and spring and winter precipitations, respectively. The allelic variation in these genes is involved in climatic adaptation. PMID: 21988878
  8. study presents evidence that the FRI (FRIGIDA) gene exhibits 'adaptive' pleiotropy, producing trait correlations along an axis that results in two adaptive strategies PMID: 23698015
  9. FRI forms a large protein complex which interacts with FLC-specific regulators and recruits cromatin modification factors. PMID: 21282526
  10. FRI and FRL1 contain a stable, central domain that is conserved across the FRI superfamily. PMID: 20405310
  11. The suppression of late flowering is caused by the inability of FRIgida to increase late flowering. PMID: 15361145
  12. The contribution to variation in flowering time and vernalization of the two genes FRIGIDA (FRI) and FLOWERING LOCUS C (FLC), previously shown to be important determinants in natural variation of flowering time is reported. PMID: 15908596
  13. FRI upregulates FLC expression that represses flowering PMID: 16547097
  14. These antagonistic pleiotropic effects reduce the adaptive value of FRI, and helps explain the maintenance of alternative life history strategies across natural populations of A. thaliana. PMID: 17940010
  15. Natural allelic variation in FRI can mediate response to selection for early flowering. PMID: 19317844
  16. FRI up-regulates FLC expression through a cotranscriptional mechanism involving direct physical interaction with the nuclear cap-binding complex with concomitant effects on FLC transcription and splicing. PMID: 19429606

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Subcellular Location
Nucleus.
Protein Families
Frigida family
Database Links

KEGG: ath:AT4G00650

STRING: 3702.AT4G00650.1

UniGene: At.16827

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