Recombinant Arabidopsis thaliana Inactive protein FRIGIDA (FRI)

Code CSB-YP727843DOA
MSDS
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Source Yeast
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Code CSB-EP727843DOA
MSDS
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Source E.coli
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Code CSB-EP727843DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP727843DOA
MSDS
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Source Baculovirus
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Code CSB-MP727843DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FRI
Uniprot No.
Alternative Names
FRI; At4g00650; F6N23.25Inactive protein FRIGIDA
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-314
Target Protein Sequence
MSNYPPTVAA QPTTTANPLL QRHQSEQRRR ELPKIVETES TSMDITIGQS KQPQFLKSID ELAAFSVAVE TFKRQFDDLQ KHIESIENAI DSKLESNGVV LAARNNNFHQ PMLSPPRNNV SVETTVTVSQ PSQEIVPETS NKPEGERICE LMCSKGLRKY IYANISDQAK LMEEIPSALK LAKEPAKFVL DCIGKFYLQG RRAFTKESPM SSARQVSLLI LESFLLMPDR GKGKVKIESW IKDEAETAAV AWRKRLMTEG GLAAAEKMDA RGLLLLVACF GVPSNFRSTD LLDLIRMSGS NEIAGALKRS QFQV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. Data indicate that FRIGIDA protein (FRI) as an activator of EARLY FLOWERING IN SHORT DAYS protein EFS (EFS) and that EFS was a histone H3 Lys36-specific methyltransferase. PMID: 28584923
  2. natural variation involving deletion alleles modulate temperature-sensitive flowering responses PMID: 26662639
  3. Structural Analysis of FRIGIDA Flowering-Time Regulator PMID: 26633860
  4. Proteasome-mediated FRI degradation regulates flowering during vernalization in Arabidopsis. PMID: 25538183
  5. Analysis of flowering time data from various Arabidopsis natural accessions indicated that the FRI-Ler allele retains some functionality. PMID: 25207670
  6. The expression of FRI gene in Arabidopsis thaliana plants in extreme conditions in northern margins of species range. PMID: 25474881
  7. The spatial patterns of FRI, FLC, and PHYC polymorphisms are significantly associated with winter temperatures and spring and winter precipitations, respectively. The allelic variation in these genes is involved in climatic adaptation. PMID: 21988878
  8. study presents evidence that the FRI (FRIGIDA) gene exhibits 'adaptive' pleiotropy, producing trait correlations along an axis that results in two adaptive strategies PMID: 23698015
  9. FRI forms a large protein complex which interacts with FLC-specific regulators and recruits cromatin modification factors. PMID: 21282526
  10. FRI and FRL1 contain a stable, central domain that is conserved across the FRI superfamily. PMID: 20405310
  11. The suppression of late flowering is caused by the inability of FRIgida to increase late flowering. PMID: 15361145
  12. The contribution to variation in flowering time and vernalization of the two genes FRIGIDA (FRI) and FLOWERING LOCUS C (FLC), previously shown to be important determinants in natural variation of flowering time is reported. PMID: 15908596
  13. FRI upregulates FLC expression that represses flowering PMID: 16547097
  14. These antagonistic pleiotropic effects reduce the adaptive value of FRI, and helps explain the maintenance of alternative life history strategies across natural populations of A. thaliana. PMID: 17940010
  15. Natural allelic variation in FRI can mediate response to selection for early flowering. PMID: 19317844
  16. FRI up-regulates FLC expression through a cotranscriptional mechanism involving direct physical interaction with the nuclear cap-binding complex with concomitant effects on FLC transcription and splicing. PMID: 19429606

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Subcellular Location
Nucleus.
Protein Families
Frigida family
Database Links

KEGG: ath:AT4G00650

STRING: 3702.AT4G00650.1

UniGene: At.16827

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