FTSH2 Antibody

Code CSB-PA528047XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FTSH2 Polyclonal antibody
Uniprot No.
Target Names
FTSH2
Alternative Names
FTSH2; VAR2; At2g30950; F7F1.16; ATP-dependent zinc metalloprotease FTSH 2, chloroplastic; AtFTSH2; Protein VARIEGATED 2
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FTSH2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Part of a complex that function as an ATP-dependent zinc metallopeptidase. Involved in the thylakoid formation and in the removal of damaged D1 in the photosystem II, preventing cell death under high-intensity light conditions, but not involved in thermotolerance.
Gene References into Functions
  1. the thylakoid membrane-bound FtsH5 and FtsH2 have dual functions in the maintenance of photosynthetic complexes. PMID: 27208291
  2. The initiation of (1)O2 signaling in grana margins depends on EX1 and the ATP-dependent zinc metalloprotease FtsH. As FtsH cleaves also the D1 protein during the disassembly of damaged PSII, EX1- and (1)O2-mediated signaling seems to be not only spatially but also functionally associated with the repair of PSII PMID: 27303039
  3. SVR10 and BPG2 are functionally related with VAR2, likely through their potential roles in regulating chloroplast protein homeostasis. PMID: 26435530
  4. A brief update of im and var2 and the functions of PTOX and AtFtsH2, is provided. PMID: 23703455
  5. The disruption of specific aspects of chloroplast translation, rather than a general impairment in chloroplast translation, suppress var2 variegation and the existence of complex genetic interactions in chloroplast development. PMID: 23721655
  6. These data demonstrate in vivo cooperative degradation of D1, in which Deg cleavage assists FtsH processive degradation under photoinhibitory conditions. PMID: 22698923
  7. To gain structural information of the possible complexes, the Arabidopsis FtsH2 (type B) and FtsH5 (type A) were modeled. PMID: 22558304
  8. Chloroplastic N-terminal methionine excision(cNME) acts upstream of the FtsH protease complex. Developmental defects and higher sensitivity to photoinhibition associated with the ftsh2 mutation were abolished when cNME was inhibited. PMID: 22010036
  9. In Arabidopsis thaliana, ATP-dependent chloroplast proteases belonging to FtsH family (especially FtsH2) are considered to be involved in aspects of chloroplast and whole plant maintenance under non-stressing as well as stressing conditions PMID: 21735825
  10. The Tat pathway integrates FtsH2 into thylakoids. PMID: 21214651
  11. Findings reinforce the strong genetic link between VAR2 and chloroplast translation, and point to a critical role of SVR3, and possibly some aspects of chloroplast translation, in the response of plants to chilling stress. PMID: 21187014
  12. The effect of FtsHs in D1 degradation was investigated in Arabidopsis (Arabidopsis thaliana) mutants lacking FtsH2 (yellow variegated2 [var2]) or FtsH5 (var1). PMID: 19767385
  13. Results showed that the conserved Gly-Ala-Asp motif plays an important role in FtsH activity and characterization of the var2 alleles could help to identify the physiologically important domain of FtsH. PMID: 15803409
  14. A model has been proposed to explain the mechanism of var2 variegation, which suggests that threshold levels of FtsH complexes are required for green sector formation. PMID: 16449381
  15. The white sectors in var2 are made by viable cells that have plastids arrested in thylakoid formation. PMID: 17449646
  16. These results indicate that photo-oxidative stress by excess light is involved in the regulation of respiratory gene expression and the modulation of respiratory properties, especially the AOX up-regulation. PMID: 18296449
  17. Data show that var2-mediated leaf variegation in Arabidopsis can be suppressed by mutations in SUPPRESSOR OF VARIEGATIONS. PMID: 18599582
  18. Chloroplast development promoted by the ectopic expression of GPA1 is dependent on FtsH2. PMID: 19228339

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Subcellular Location
Plastid, chloroplast thylakoid membrane; Single-pass membrane protein; Stromal side.
Protein Families
AAA ATPase family; Peptidase M41 family
Tissue Specificity
Expressed in cotyledons, cauline and rosette leaves, stems, sepals, flovers and siliques. Very low in roots.
Database Links

KEGG: ath:AT2G30950

STRING: 3702.AT2G30950.1

UniGene: At.22024

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