Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FTSZ2-1 Polyclonal antibody

Uniprot No.

O82533

Target Names

FTSZ2-1

Alternative Names

FTSZ2-1 antibody; At2g36250 antibody; F2H17.14Cell division protein FtsZ homolog 2-1 antibody; chloroplastic antibody; AtFtsZ2-1 antibody; Plastid division protein FTSZ2-1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana FTSZ2-1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Exhibits GTPase activity. Component of the plastid division machinery that forms a contractile ring at the division site. Required for plastid division in a dose-dependent manner.

Gene References into Functions

Chloroplast division protein ARC3 acts on FtsZ2 by preventing filament bundling and enhancing GTPase activity. PMID: 29138260
FtsZ2-2, generally resulted in stronger effects in the shoot apex than those observed in mature leaves. The implications of these findings, along with additional observations made in this work, to our understanding of the mechanisms and regulation of plastid proliferation in the shoot apex are discussed. PMID: 29920253
FtsZ1 and FtsZ2 can heteropolymerize into a contractible ring ex vivo. Fluorescently labelled FtsZ1 and/or FtsZ2 formed single rings in cells of the yeast Pichia pastoris. Photobleaching experiments indicated that co-assembly of FtsZ1 and FtsZ2 imparts polarity to polymerization. PMID: 27322658
The negative regulation of FtsZ assembly by PARC6 PMID: 26527658
In plants lacking ARC3, the key negative regulator of FtsZ assembly, FtsZ1/FtsZ2 turnover was threefold slower. PMID: 25731613
Results suggest that ARC3 directly inhibits Z-ring assembly through interaction with FtsZ2 in heteropolymers and that ARC3 activity is spatially regulated by MinD1 and MinE1 to permit Z-ring assembly at the mid-plastid. PMID: 23715471
FtsZ1 and FtsZ2-1 are capable of in vitro self-assembly into two distinct filament types, termed type-I and type-II. During assembly of the type-II filaments the most common intermediate between the dimer and filament state is a tetramer. PMID: 21781955
Data show that FtsZ2-1 is essential for chloroplast division and that FtsZ2-2 plays a specific role in chloroplast morphology and internal organisation in addition to participating in chloroplast partition. PMID: 21439281
Specific GTPase activities were determined and found to be different for FtsZ1-1 vs. FtsZ2-1. PMID: 19925792
Results describe the in vivo quantitative relationship between plastid division proteins FtsZ1 and FtsZ2 and identification of ARC6 and ARC3 in a native FtsZ complex. PMID: 18284374

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Subcellular Location

Plastid, chloroplast stroma. Plastid, chloroplast thylakoid membrane; Peripheral membrane protein. Note=Forms a contractile ring at the chloroplast midpoint that coaligns with FTSZ1 rings.

Protein Families

FtsZ family

Database Links

KEGG: ath:AT2G36250

STRING: 3702.AT2G36250.1

UniGene: At.14112

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FTSZ2-1 Antibody

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