Recombinant Arabidopsis thaliana Cell division protein FtsZ homolog 2-1, chloroplastic (FTSZ2-1), partial

Code CSB-YP526873DOA
MSDS
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Source Yeast
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Code CSB-EP526873DOA
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Source E.coli
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Code CSB-EP526873DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP526873DOA
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Source Baculovirus
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Code CSB-MP526873DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FTSZ2-1
Uniprot No.
Alternative Names
FTSZ2-1; At2g36250; F2H17.14Cell division protein FtsZ homolog 2-1; chloroplastic; AtFtsZ2-1; Plastid division protein FTSZ2-1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
-
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Exhibits GTPase activity. Component of the plastid division machinery that forms a contractile ring at the division site. Required for plastid division in a dose-dependent manner.
Gene References into Functions
  1. Chloroplast division protein ARC3 acts on FtsZ2 by preventing filament bundling and enhancing GTPase activity. PMID: 29138260
  2. FtsZ2-2, generally resulted in stronger effects in the shoot apex than those observed in mature leaves. The implications of these findings, along with additional observations made in this work, to our understanding of the mechanisms and regulation of plastid proliferation in the shoot apex are discussed. PMID: 29920253
  3. FtsZ1 and FtsZ2 can heteropolymerize into a contractible ring ex vivo. Fluorescently labelled FtsZ1 and/or FtsZ2 formed single rings in cells of the yeast Pichia pastoris. Photobleaching experiments indicated that co-assembly of FtsZ1 and FtsZ2 imparts polarity to polymerization. PMID: 27322658
  4. The negative regulation of FtsZ assembly by PARC6 PMID: 26527658
  5. In plants lacking ARC3, the key negative regulator of FtsZ assembly, FtsZ1/FtsZ2 turnover was threefold slower. PMID: 25731613
  6. Results suggest that ARC3 directly inhibits Z-ring assembly through interaction with FtsZ2 in heteropolymers and that ARC3 activity is spatially regulated by MinD1 and MinE1 to permit Z-ring assembly at the mid-plastid. PMID: 23715471
  7. FtsZ1 and FtsZ2-1 are capable of in vitro self-assembly into two distinct filament types, termed type-I and type-II. During assembly of the type-II filaments the most common intermediate between the dimer and filament state is a tetramer. PMID: 21781955
  8. Data show that FtsZ2-1 is essential for chloroplast division and that FtsZ2-2 plays a specific role in chloroplast morphology and internal organisation in addition to participating in chloroplast partition. PMID: 21439281
  9. Specific GTPase activities were determined and found to be different for FtsZ1-1 vs. FtsZ2-1. PMID: 19925792
  10. Results describe the in vivo quantitative relationship between plastid division proteins FtsZ1 and FtsZ2 and identification of ARC6 and ARC3 in a native FtsZ complex. PMID: 18284374

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Subcellular Location
Plastid, chloroplast stroma. Plastid, chloroplast thylakoid membrane; Peripheral membrane protein. Note=Forms a contractile ring at the chloroplast midpoint that coaligns with FTSZ1 rings.
Protein Families
FtsZ family
Database Links

KEGG: ath:AT2G36250

STRING: 3702.AT2G36250.1

UniGene: At.14112

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