Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GATA18 Polyclonal antibody

Uniprot No.

Q8LC79

Target Names

GATA18

Alternative Names

GATA18 antibody; HAN antibody; At3g50870 antibody; F18B3.150 antibody; GATA transcription factor 18 antibody; Protein HANABA TARANU antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana GATA18 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcriptional factor that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters (including its own promoter and GATA21 promoter), thus regulating the expression of genes mostly involved in hormone responses and floral organ specification (including genes regulating hormones responses). Regulates both flower and shoot apical meristem (SAM) development, especially for establishing organ boundaries in shoots and flowers, probably by controlling the number and position of WUS-expressing cells. Coregulates, with AGO10/PNH, the shoot apical meristem (SAM) organization. Regulates floral organ development via the promotion of JAG and NPR5/BOP2 expression. Modulates cytokinin homeostasis in organ boundaries by regulating CKX3 expression. Involved in cell proliferation and differentiation. Required to position the inductive proembryo boundary via the regulation of gene expression and for early embryonic development. Together with GIF1/AN3, mediates cotyledon identity by preventing ectopic root formation through the repression of PLT1 expression.

Gene References into Functions

HAN functions as a key repressor that regulates floral development via regulatory networks involving genes in the GATA3 family, along with genes involved in hormone action and floral organ specification. PMID: 23335616
The data suggested that establishing cotyledon identity requires both GIF1 and HANABA TARANU to repress ectopic root formation by repressing PLETHORA1 expression. PMID: 22669825
Mutations in HAN cause a coordinated apical shift of gene expression patterns, revealing that HAN regulates transcription in the basal proembryo. [GATA factor HANABA TARANU] PMID: 20643354

Subcellular Location

Nucleus.

Protein Families

Type IV zinc-finger family, Class B subfamily

Tissue Specificity

Expressed in vegetative and inflorescence shoot apical meristems (SAMs), axillary (SAMs), floral meristems, developing ovules and stamens, vascular tissues, and in the embryo.

Database Links

KEGG: ath:AT3G50870

STRING: 3702.AT3G50870.1

UniGene: At.27761

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GATA18 Antibody

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