Recombinant Arabidopsis thaliana GATA transcription factor 18 (GATA18)

Code CSB-YP814500DOA
MSDS
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Source Yeast
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Code CSB-EP814500DOA
MSDS
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Source E.coli
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Code CSB-EP814500DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP814500DOA
MSDS
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Source Baculovirus
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Code CSB-MP814500DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GATA18
Uniprot No.
Alternative Names
GATA18; HAN; At3g50870; F18B3.150; GATA transcription factor 18; Protein HANABA TARANU
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-295
Target Protein Sequence
MMQTPYTTST QGQYCHSCGM FHHHSQSCCY NNNNNSNAGS YSMVFSMQNG GVFEQNGEDY HHSSSLVDCT LSLGTPSTRL CEEDEKRRRS TSSGASSCIS NFWDLIHTKN NNSKTAPYNN VPSFSANKPS RGCSGGGGGG GGGGGGDSLL ARRCANCDTT STPLWRNGPR GPKSLCNACG IRFKKEERRT TAATGNTVVG AAPVQTDQYG HHNSGYNNYH AATNNNNNNG TPWAHHHSTQ RVPCNYPANE IRFMDDYGSG VANNVESDGA HGGVPFLSWR LNVADRASLV HDFTR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional factor that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters (including its own promoter and GATA21 promoter), thus regulating the expression of genes mostly involved in hormone responses and floral organ specification (including genes regulating hormones responses). Regulates both flower and shoot apical meristem (SAM) development, especially for establishing organ boundaries in shoots and flowers, probably by controlling the number and position of WUS-expressing cells. Coregulates, with AGO10/PNH, the shoot apical meristem (SAM) organization. Regulates floral organ development via the promotion of JAG and NPR5/BOP2 expression. Modulates cytokinin homeostasis in organ boundaries by regulating CKX3 expression. Involved in cell proliferation and differentiation. Required to position the inductive proembryo boundary via the regulation of gene expression and for early embryonic development. Together with GIF1/AN3, mediates cotyledon identity by preventing ectopic root formation through the repression of PLT1 expression.
Gene References into Functions
  1. HAN functions as a key repressor that regulates floral development via regulatory networks involving genes in the GATA3 family, along with genes involved in hormone action and floral organ specification. PMID: 23335616
  2. The data suggested that establishing cotyledon identity requires both GIF1 and HANABA TARANU to repress ectopic root formation by repressing PLETHORA1 expression. PMID: 22669825
  3. Mutations in HAN cause a coordinated apical shift of gene expression patterns, revealing that HAN regulates transcription in the basal proembryo. [GATA factor HANABA TARANU] PMID: 20643354
Subcellular Location
Nucleus.
Protein Families
Type IV zinc-finger family, Class B subfamily
Tissue Specificity
Expressed in vegetative and inflorescence shoot apical meristems (SAMs), axillary (SAMs), floral meristems, developing ovules and stamens, vascular tissues, and in the embryo.
Database Links

KEGG: ath:AT3G50870

STRING: 3702.AT3G50870.1

UniGene: At.27761

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