GCR1 Antibody

Code CSB-PA009728XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GCR1 Polyclonal antibody
Uniprot No.
Target Names
GCR1
Alternative Names
GCR1; At1g48270; F11A17.17; G-protein coupled receptor 1
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GCR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Together with GPA1, may regulate the cell cycle via a signaling cascade that uses phosphatidylinositol-specific phospholipase C (PI-PLC) as an effector and inositol 1,4,5-trisphosphate(IP(3)) as a second messenger. Promotes PI-PLC activity and IP(3) accumulation. Involved in the blue light (BL) signaling. Together with GPA1 and ADT3, required for BL-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe), in etiolated seedlings. Probably involved in cytokinin signal transduction. Plays a positive role in gibberellin- (GA) and brassinosteroid- (BR) regulated seed germination, probably independently of a heterotrimeric G-protein. Mediates seed dormancy abolition, and promotes seed germination and flowering.
Gene References into Functions
  1. provide compelling genetic evidence to revisit the role of GCR1 in G-protein signalling PMID: 26346778
  2. GCR1 may act as a negative regulator of G protein alpha subunit GPA1-mediated abscisic acid responses in guard cells [GCR1] PMID: 15155892
  3. GCR1 plays a positive role in gibberellin and brassinosteroid regulated seed germination. Unlike a typical 7TM receptor, it acts independently of the heterotrimeric G-protein. PMID: 15181210
Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=Localized to the outer edge of the leaf epidermal cells in a punctuate pattern.
Protein Families
G-protein coupled receptor 2 family
Tissue Specificity
Mostly present in the meristematic regions. Expressed at low levels in seedlings, vascular tissues of cotyledons, hypocotyl, and roots, stems, leaves, flowering buds and siliques. In dark-grown seedlings, localized in the cotyledons and the hook.
Database Links

KEGG: ath:AT1G48270

STRING: 3702.AT1G48270.1

UniGene: At.21469

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