VTC2 Antibody

Code CSB-PA823310XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) VTC2 Polyclonal antibody
Uniprot No.
Target Names
VTC2
Alternative Names
VTC2 antibody; At4g26850 antibody; F10M23.190GDP-L-galactose phosphorylase 1 antibody; EC 2.7.7.69 antibody; Protein VITAMIN C DEFECTIVE 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana VTC2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Acts as a phosphorylase rather than as a transferase. Uses preferentially GDP-L-galactose and GDP-D-glucose as substrates. Lower activity with GDP-L-fucose, very low activity with GDP-D-mannose, and no activity with UDP-D-glucose, UDP-D-galactose or ADP-D-glucose. Highly specific for inorganic phosphate as the guanylyl acceptor.
Gene References into Functions
  1. GGP/VTC2 acts as a rate-limiting step regulating AsA biosynthesis in response to light and dark conditions PMID: 25036484
  2. The VTC5 promoter is more active in young cotyledons; the VTC2 and VTC5 promoters for two GDP-L-galactose phosphorylase isozymes drive a day-to-night variation in expression. PMID: 21897033
  3. A high proportion of the primary roots of the vtc2 plants grown on soil had lost the wild-type responses to gravity. PMID: 16720601
  4. VTC2 from A. thaliana is a specific GDP-L-galactose/GDP-D-glucose phosphorylase PMID: 17462988
  5. At4g26850 codes for GDP-L-galactose-hexose-1-phosphate guanyltransferase which catalyzing the transfer of GMP from GDP-L-galactose to a hexose-1-P, most likely D-mannose-1-phosphate in vivo. [galactose guanyltransferase] PMID: 17485667
  6. Data show that a GDP-L-galactose phosphorylase, encoded by the Arabidopsis thaliana VTC2 gene, catalyses this step in the ascorbate biosynthetic pathway. PMID: 17877701
  7. Arabidopsis VTC2 and VTC5 proteins are enzymes that guanylylate a conserved active site His residue with GDP-L-galactose, forming L-galactose 1-phosphate for vitamin C synthesis, and regenerate the enzyme with phosphate to form GDP. PMID: 18463094
  8. The VTC2 gene was expressed in all developmental stages, and the mRNA was expressed at a higher level in green tissues than in the root. PMID: 18516687

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
GDPGP1 family
Tissue Specificity
Expressed in leaves, stems, roots, flowers and siliques. Highest expression in green tissues.
Database Links

KEGG: ath:AT4G26850

STRING: 3702.AT4G26850.1

UniGene: At.23783

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