Product Details

Purity

>85% (SDS-PAGE)

Target Names

VTC2

Uniprot No.

Q8RWE8

Alternative Names

VTC2; At4g26850; F10M23.190GDP-L-galactose phosphorylase 1; EC 2.7.7.69; Protein VITAMIN C DEFECTIVE 2

Species

Arabidopsis thaliana (Mouse-ear cress)

Expression Region

1-442

Target Protein Sequence

MLKIKRVPTV VSNYQKDDGA EDPVGCGRNC LGACCLNGAR LPLYACKNLV KSGEKLVISH EAIEPPVAFL ESLVLGEWED RFQRGLFRYD VTACETKVIP GKYGFVAQLN EGRHLKKRPT EFRVDKVLQS FDGSKFNFTK VGQEELLFQF EAGEDAQVQF FPCMPIDPEN SPSVVAINVS PIEYGHVLLI PRVLDCLPQR IDHKSLLLAV HMAAEAANPY FRLGYNSLGA FATINHLHFQ AYYLAMPFPL EKAPTKKITT TVSGVKISEL LSYPVRSLLF EGGSSMQELS DTVSDCCVCL QNNNIPFNIL ISDCGRQIFL MPQCYAEKQA LGEVSPEVLE TQVNPAVWEI SGHMVLKRKE DYEGASEDNA WRLLAEASLS EERFKEVTAL AFEAIGCSNQ EEDLEGTIVH QQNSSGNVNQ KSNRTHGGPI TNGTAAECLV LQ

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Acts as a phosphorylase rather than as a transferase. Uses preferentially GDP-L-galactose and GDP-D-glucose as substrates. Lower activity with GDP-L-fucose, very low activity with GDP-D-mannose, and no activity with UDP-D-glucose, UDP-D-galactose or ADP-D-glucose. Highly specific for inorganic phosphate as the guanylyl acceptor.

Gene References into Functions

GGP/VTC2 acts as a rate-limiting step regulating AsA biosynthesis in response to light and dark conditions PMID: 25036484
The VTC5 promoter is more active in young cotyledons; the VTC2 and VTC5 promoters for two GDP-L-galactose phosphorylase isozymes drive a day-to-night variation in expression. PMID: 21897033
A high proportion of the primary roots of the vtc2 plants grown on soil had lost the wild-type responses to gravity. PMID: 16720601
VTC2 from A. thaliana is a specific GDP-L-galactose/GDP-D-glucose phosphorylase PMID: 17462988
At4g26850 codes for GDP-L-galactose-hexose-1-phosphate guanyltransferase which catalyzing the transfer of GMP from GDP-L-galactose to a hexose-1-P, most likely D-mannose-1-phosphate in vivo. [galactose guanyltransferase] PMID: 17485667
Data show that a GDP-L-galactose phosphorylase, encoded by the Arabidopsis thaliana VTC2 gene, catalyses this step in the ascorbate biosynthetic pathway. PMID: 17877701
Arabidopsis VTC2 and VTC5 proteins are enzymes that guanylylate a conserved active site His residue with GDP-L-galactose, forming L-galactose 1-phosphate for vitamin C synthesis, and regenerate the enzyme with phosphate to form GDP. PMID: 18463094
The VTC2 gene was expressed in all developmental stages, and the mRNA was expressed at a higher level in green tissues than in the root. PMID: 18516687

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

GDPGP1 family

Tissue Specificity

Expressed in leaves, stems, roots, flowers and siliques. Highest expression in green tissues.

Database Links

KEGG: ath:AT4G26850

STRING: 3702.AT4G26850.1

UniGene: At.23783

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Recombinant Arabidopsis thaliana GDP-L-galactose phosphorylase 1 (VTC2)

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