Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GH3.12 Polyclonal antibody

Uniprot No.

Q9LYU4

Target Names

GH3.12

Alternative Names

GH3.12 antibody; GDG1 antibody; PBS3 antibody; WIN3 antibody; At5g13320 antibody; T22N19.5 antibody; T31B5.1404-substituted benzoates-glutamate ligase GH3.12 antibody; EC 6.3.2.- antibody; Auxin-responsive GH3-like protein 12 antibody; AtGH3-12 antibody; Protein GH3-LIKE DEFENSE GENE 1 antibody; Protein GRETCHEN HAGEN 3.12 antibody; Protein HOPW1-1-INTERACTING 3 antibody; Protein avrPPHB SUSCEPTIBLE 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana GH3.12 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).

Gene References into Functions

The X-ray crystal structure of GH3.12 is presented in the closed conformation in complex with the nonhydrolysable ATP analogue AMPCPP and the substrate salicylate. PMID: 24100325
WIN3 represents a novel node in the salicylic acid signaling networks to regulate plant defense and flowering time. [WIN3] PMID: 21543726
Amino acid conjugation plays a critical role in salicylic acid (SA) metabolism and induced defense responses, with PBS3 acting upstream of SA, directly on SA, or on a competitive inhibitor of SA. PMID: 17468220
Molecular characterization of the GH3-LIKE DEFENSE GENE 1 (GDG1), a member of the GH3-like gene family, shows that GDG1 is an important component of salicylic acid mediated defense against the bacterial pathogen P. syringae.[GDG1] PMID: 17521413
WIN3 (At5g13320) is a key element of the RPS2 defense response pathway and a basal and systemic defense component. PMID: 17918621
WIN3 is partially required for Pseudomonas syringae HopW1-1-induced disease resistance. PMID: 18266921
Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate PMID: 19189963

Hide All

Protein Families

IAA-amido conjugating enzyme family

Tissue Specificity

Expressed in seedlings, mostly in cotyledons, leaves, hypocotyls and sporadically in roots. Not detected in unchallenged adult plants, except in flowers.

Database Links

KEGG: ath:AT5G13320

STRING: 3702.AT5G13320.1

UniGene: At.32084

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GH3.12 Antibody

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