Recombinant Arabidopsis thaliana 4-substituted benzoates-glutamate ligase GH3.12 (GH3.12), partial

Code CSB-YP878640DOA
MSDS
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Source Yeast
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Code CSB-EP878640DOA
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Source E.coli
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Code CSB-EP878640DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878640DOA
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Source Baculovirus
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Code CSB-MP878640DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GH3.12
Uniprot No.
Alternative Names
GH3.12; GDG1; PBS3; WIN3; At5g13320; T22N19.5; T31B5.1404-substituted benzoates-glutamate ligase GH3.12; EC 6.3.2.-; Auxin-responsive GH3-like protein 12; AtGH3-12; Protein GH3-LIKE DEFENSE GENE 1; Protein GRETCHEN HAGEN 3.12; Protein HOPW1-1-INTERACTING 3; Protein avrPPHB SUSCEPTIBLE 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).
Gene References into Functions
  1. The X-ray crystal structure of GH3.12 is presented in the closed conformation in complex with the nonhydrolysable ATP analogue AMPCPP and the substrate salicylate. PMID: 24100325
  2. WIN3 represents a novel node in the salicylic acid signaling networks to regulate plant defense and flowering time. [WIN3] PMID: 21543726
  3. Amino acid conjugation plays a critical role in salicylic acid (SA) metabolism and induced defense responses, with PBS3 acting upstream of SA, directly on SA, or on a competitive inhibitor of SA. PMID: 17468220
  4. Molecular characterization of the GH3-LIKE DEFENSE GENE 1 (GDG1), a member of the GH3-like gene family, shows that GDG1 is an important component of salicylic acid mediated defense against the bacterial pathogen P. syringae.[GDG1] PMID: 17521413
  5. WIN3 (At5g13320) is a key element of the RPS2 defense response pathway and a basal and systemic defense component. PMID: 17918621
  6. WIN3 is partially required for Pseudomonas syringae HopW1-1-induced disease resistance. PMID: 18266921
  7. Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate PMID: 19189963

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Protein Families
IAA-amido conjugating enzyme family
Tissue Specificity
Expressed in seedlings, mostly in cotyledons, leaves, hypocotyls and sporadically in roots. Not detected in unchallenged adult plants, except in flowers.
Database Links

KEGG: ath:AT5G13320

STRING: 3702.AT5G13320.1

UniGene: At.32084

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