Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) glnK Polyclonal antibody

Uniprot No.

P0AC55

Target Names

glnK

Alternative Names

glnK antibody; ybaI antibody; b0450 antibody; JW0440Nitrogen regulatory protein P-II 2 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) glnK protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in the regulation of nitrogen metabolism. Regulates the activity of its targets by protein-protein interaction in response to the nitrogen status of the cell. Involved in the regulation of the ammonium transporter AmtB so as to optimize ammonium uptake under all growth conditions. In nitrogen-limited conditions, GlnK does not interact with AmtB, which remains active and imports ammonium. When extracellular ammonium increases, GlnK associates tightly with AmtB in the inner membrane, thereby inhibiting the transporter activity. Also involved in the regulation of the glutamine synthetase adenylyltransferase/adenylyl-removing (AT/AR) enzyme GlnE and the glutamine synthetase GlnA. In nitrogen-limited conditions, formation of uridylylated GlnB(PII)/GlnK heterotrimers may fine-regulate the activation of GlnA: uridylylated heterotrimers stimulate the deadenylation and the activation of GlnA whereas uridylylated GlnK homotrimers do not stimulate, or hardly stimulate, the deadenylation of GlnA. In addition, regulates the expression of Ntr genes during nitrogen starvation, probably via the control of the levels of phosphorylated NRI during different stages of the response to nitrogen limitation.

Gene References into Functions

Results show that GlnK is tightly regulated under nitrogen-rich conditions, yet it is expressed during ammonium run-out and starvation. This suggests a role for GlnK as a buffer of nitrogen shock after starvation, and provides a further functional link between nitrogen and carbon metabolisms. PMID: 28538158
Data show that the P(II) protein, GlnK, has an ATPase activity that is inhibited by 2-oxoglutarate. PMID: 23818625
Studies suggest a hypothesise that the regulatory protein GlnK is required to fine-tune the active transport of ammonium in order to limit futile cycling whilst enabling an intracellular ammonium level sufficient for the cell's nitrogen requirements. PMID: 21134373
Co-purification of GlnK with AmtB from ammonium-shocked E. coli cells definitvely demonstrates that these two proteins form a functional complex with a stoichiometry of 1:1 and dependent on the uridylylation status of GlnK. PMID: 16864585
Data show a subtle interplay between GlnB and GlnK. PMID: 19438718

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Subcellular Location

Cytoplasm. Cell inner membrane.

Protein Families

P(II) protein family

Database Links

KEGG: ecj:JW0440

STRING: 316385.ECDH10B_0406

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glnK Antibody

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