CYT1 Antibody

Code CSB-PA519497XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CYT1 Polyclonal antibody
Uniprot No.
Target Names
CYT1
Alternative Names
CYT1 antibody; EMB101 antibody; GMP1 antibody; HSN1 antibody; SOZ1 antibody; VTC1 antibody; At2g39770 antibody; T5I7.7Mannose-1-phosphate guanylyltransferase 1 antibody; EC 2.7.7.13 antibody; GDP-mannose pyrophosphorylase 1 antibody; Protein CYTOKINESIS DEFECTIVE 1 antibody; Protein EMBRYO DEFECTIVE 101 antibody; Protein HYPERSENSITIVE TO AMMONIUM ION 1 antibody; Protein SENSITIVE TO OZONE 1 antibody; Protein VITAMIN C DEFECTIVE 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CYT1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Plays an essential role in plant growth and development and cell-wall architecture. Provides GDP-mannose, used for cell wall carbohydrate biosynthesis, protein N-glycosylation, as well as for the biosynthesis of the antioxidant ascorbate.
Gene References into Functions
  1. Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1 have been described. PMID: 27710946
  2. Data reveal that the 27th amino acid of VTC1 confers a key regulation in the interaction with CSN5B and AsA biosynthesis, as well as in Arabidopsis seedling growth. PMID: 27561782
  3. VTC1 is degraded in the dark through the 26S proteasome pathway, and this degradation requires CSN5B. PMID: 23424245
  4. Arabidopsis vtc1 and vtc2 mutants, that have low levels of ascorbate, were more susceptible to Alternaria brassicicola. PMID: 23134520
  5. A mutation in vtc1 causes hypersensitivity to ammonium, resulting in root growth inhibition, altered ammonium metabolism, and hormone homeostasis. PMID: 20007685
  6. Programmed cell death symptoms such as nuclear chromatin condensation, the presence of multivesicular bodies, and extensive degradation and disorganization of the grana stacks were observed in 10-week-old vtc1 leaves. PMID: 16720601

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Transferase hexapeptide repeat family
Database Links

KEGG: ath:AT2G39770

STRING: 3702.AT2G39770.1

UniGene: At.10348

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