Recombinant Arabidopsis thaliana Mannose-1-phosphate guanylyltransferase 1 (CYT1)

Code CSB-YP519497DOA
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Source Yeast
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Code CSB-EP519497DOA
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Source E.coli
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Code CSB-EP519497DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519497DOA
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Source Baculovirus
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Code CSB-MP519497DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYT1
Uniprot No.
Alternative Names
CYT1; EMB101; GMP1; HSN1; SOZ1; VTC1; At2g39770; T5I7.7Mannose-1-phosphate guanylyltransferase 1; EC 2.7.7.13; GDP-mannose pyrophosphorylase 1; Protein CYTOKINESIS DEFECTIVE 1; Protein EMBRYO DEFECTIVE 101; Protein HYPERSENSITIVE TO AMMONIUM ION 1; Protein SENSITIVE TO OZONE 1; Protein VITAMIN C DEFECTIVE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-361
Target Protein Sequence
MKALILVGGF GTRLRPLTLS FPKPLVDFAN KPMILHQIEA LKAVGVDEVV LAINYQPEVM LNFLKDFETK LEIKITCSQE TEPLGTAGPL ALARDKLLDG SGEPFFVLNS DVISEYPLKE MLEFHKSHGG EASIMVTKVD EPSKYGVVVM EESTGRVEKF VEKPKLYVGN KINAGIYLLN PSVLDKIELR PTSIEKETFP KIAAAQGLYA MVLPGFWMDI GQPRDYITGL RLYLDSLRKK SPAKLTSGPH IVGNVLVDET ATIGEGCLIG PDVAIGPGCI VESGVRLSRC TVMRGVRIKK HACISSSIIG WHSTVGQWAR IENMTILGED VHVSDEIYSN GGVVLPHKEI KSNILKPEIV M
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Plays an essential role in plant growth and development and cell-wall architecture. Provides GDP-mannose, used for cell wall carbohydrate biosynthesis, protein N-glycosylation, as well as for the biosynthesis of the antioxidant ascorbate.
Gene References into Functions
  1. Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1 have been described. PMID: 27710946
  2. Data reveal that the 27th amino acid of VTC1 confers a key regulation in the interaction with CSN5B and AsA biosynthesis, as well as in Arabidopsis seedling growth. PMID: 27561782
  3. VTC1 is degraded in the dark through the 26S proteasome pathway, and this degradation requires CSN5B. PMID: 23424245
  4. Arabidopsis vtc1 and vtc2 mutants, that have low levels of ascorbate, were more susceptible to Alternaria brassicicola. PMID: 23134520
  5. A mutation in vtc1 causes hypersensitivity to ammonium, resulting in root growth inhibition, altered ammonium metabolism, and hormone homeostasis. PMID: 20007685
  6. Programmed cell death symptoms such as nuclear chromatin condensation, the presence of multivesicular bodies, and extensive degradation and disorganization of the grana stacks were observed in 10-week-old vtc1 leaves. PMID: 16720601

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Transferase hexapeptide repeat family
Database Links

KEGG: ath:AT2G39770

STRING: 3702.AT2G39770.1

UniGene: At.10348

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