Galphao Antibody

Code CSB-PA325422XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Galphao Polyclonal antibody
Uniprot No.
Target Names
Galphao
Alternative Names
Galphao antibody; G-oa47A antibody; G-oalpha47A antibody; CG2204G protein alpha o subunit antibody; Guanine nucleotide-binding protein G(o) subunit alpha 47A antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Galphao protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Plays a role in glial cell differentiation during embryogenesis; loco, Galphai and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord.
Gene References into Functions
  1. We have taken advantage of this approach to stabilize protein G alpha-go from Drosophila melanogaster. The designed SS-bridge increased by 4 degrees the melting temperature of one domain of protein G alpha-go . PMID: 26786871
  2. The heterotrimeric G protein Go functions as a transducer of Wingless-Frizzled 2 signaling in the synapse and Ankyrin 2 as a target of Go signaling required for neuromuscular junction formation. PMID: 25139856
  3. Galphao47A is required in bitter-sensitive taste neurons for L-canavanine sensitivity. PMID: 23671680
  4. Go is required for maximal physiological responses to multiple odorants in Drosophila, and olfactory receptor channel function and G-protein signaling are required for odor responsiveness. PMID: 19943954
  5. The roles for Galpha(o) subunit (Go) in mediating the two distinct pathways transduced by Fz receptors in Drosophila. PMID: 15652486

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Protein Families
G-alpha family, G(i/o/t/z) subfamily
Tissue Specificity
Expressed primarily in neuronal cell bodies in the brain, optic lobe, and thoracic and abdominal ganglia. Also expressed in antenna, oocytes and ovarian nurse cells.
Database Links

KEGG: dme:Dmel_CG2204

STRING: 7227.FBpp0087359

UniGene: Dm.12982

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