Recombinant Drosophila melanogaster Guanine nucleotide-binding protein G (o) subunit alpha 47A

Code CSB-YP325422DLU
MSDS
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Source Yeast
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Code CSB-EP325422DLU
MSDS
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Source E.coli
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Code CSB-EP325422DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP325422DLU
MSDS
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Source Baculovirus
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Code CSB-MP325422DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Galphao
Uniprot No.
Alternative Names
Galphao; G-oa47A; G-oalpha47A; CG2204G protein alpha o subunit; Guanine nucleotide-binding protein G(o) subunit alpha 47A
Species
Drosophila melanogaster (Fruit fly)
Expression Region
2-354
Target Protein Sequence
GCAQSAEER AAAARSRLIE RNLKEDGIQA AKDIKLLLLG AGESGKSTIV KQMKIIHESG FTAEDFKQYR PVVYSNTIQS LVAILRAMPT LSIQYSNNER ESDAKMVFDV CQRMHDTEPF SEELLAAMKR LWQDAGVQEC FSRSNEYQLN DSAKYFLDDL DRLGAKDYQP TEQDILRTRV KTTGIVEVHF SFKNLNFKLF DVGGQRSERK KWIHCFEDVT AIIFCVAMSE YDQVLHEDET TNRMQESLKL FDSICNNKWF TDTSIILFLN KKDLFEEKIR KSPLTICFPE YTGGQEYGEA AAYIQAQFEA KNKSTSKEIY CHMTCATDTN NIQFVFDAVT DVIIANNLRG CGLY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Plays a role in glial cell differentiation during embryogenesis; loco, Galphai and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord.
Gene References into Functions
  1. We have taken advantage of this approach to stabilize protein G alpha-go from Drosophila melanogaster. The designed SS-bridge increased by 4 degrees the melting temperature of one domain of protein G alpha-go . PMID: 26786871
  2. The heterotrimeric G protein Go functions as a transducer of Wingless-Frizzled 2 signaling in the synapse and Ankyrin 2 as a target of Go signaling required for neuromuscular junction formation. PMID: 25139856
  3. Galphao47A is required in bitter-sensitive taste neurons for L-canavanine sensitivity. PMID: 23671680
  4. Go is required for maximal physiological responses to multiple odorants in Drosophila, and olfactory receptor channel function and G-protein signaling are required for odor responsiveness. PMID: 19943954
  5. The roles for Galpha(o) subunit (Go) in mediating the two distinct pathways transduced by Fz receptors in Drosophila. PMID: 15652486

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Protein Families
G-alpha family, G(i/o/t/z) subfamily
Tissue Specificity
Expressed primarily in neuronal cell bodies in the brain, optic lobe, and thoracic and abdominal ganglia. Also expressed in antenna, oocytes and ovarian nurse cells.
Database Links

KEGG: dme:Dmel_CG2204

STRING: 7227.FBpp0087359

UniGene: Dm.12982

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