gon-1 Antibody

Code CSB-PA619308XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans gon-1 Polyclonal antibody
Uniprot No.
Target Names
gon-1
Alternative Names
gon-1 antibody; F25H8.3A disintegrin and metalloproteinase with thrombospondin motifs gon-1 antibody; ADAMTS gon-1 antibody; EC 3.4.24.- antibody; Abnormal gonad development protein 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans gon-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Secreted metalloprotease required for distal tip cell (DTC) migration along the body wall basement membranes, a key step that promotes gonad morphogenesis. Probably acts by remodeling the basement membrane during cell migration. Required to restrict presynaptic growth at the neuromuscular junctions (NMJ) in late larval stage and in adult motor neurons, probably by controlling collagen IV emb-9 degradation, a component of the synapse basement membrane. Also involved in the organization of adult muscle morphology. Has a protease-independent function in promoting the transport from the endoplasmic reticulum to the Golgi apparatus of a variety of secretory cargos. Required for the secretion of insulin-like peptide ins-7, daf-28 and ins-18 and TGF beta-like protein daf-7. In peripheral tissues, negatively regulates insulin-mediated daf-16 translocation and thereby negatively regulates lifespan and dauer formation.
Gene References into Functions
  1. propose that GON-1 regulates a developmental switch out of an initial "pro-growth" phase during which muscle arms grow out and form synapses with motor neuron axons. PMID: 25232106
  2. ADAMTS9 and GON-1 in the ER that promotes protein transport from the ER to the Golgi. This function is GON-domain dependent but protease activity independent. PMID: 22419820
  3. GON-1 has human homologues that are in the same protease subfamily PMID: 12514189
Subcellular Location
Secreted, extracellular space, extracellular matrix, basement membrane. Endoplasmic reticulum. Golgi apparatus.
Tissue Specificity
Expressed by the gonadal distal tip cells (DTCs). Expressed in muscles, including body wall, vulval and anal depressor muscles. Expressed in motor neurons and in ASI and ASJ neurons.
Database Links

KEGG: cel:CELE_F25H8.3

STRING: 6239.F25H8.3b

UniGene: Cel.8168

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