Recombinant A disintegrin and metalloproteinase with thrombospondin motifs gon-1 (gon-1), partial

Code CSB-YP619308CXY
MSDS
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Source Yeast
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Code CSB-EP619308CXY
MSDS
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Source E.coli
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Code CSB-EP619308CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619308CXY
MSDS
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Source Baculovirus
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Code CSB-MP619308CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gon-1
Uniprot No.
Alternative Names
gon-1; F25H8.3A disintegrin and metalloproteinase with thrombospondin motifs gon-1; ADAMTS gon-1; EC 3.4.24.-; Abnormal gonad development protein 1
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Secreted metalloprotease required for distal tip cell (DTC) migration along the body wall basement membranes, a key step that promotes gonad morphogenesis. Probably acts by remodeling the basement membrane during cell migration. Required to restrict presynaptic growth at the neuromuscular junctions (NMJ) in late larval stage and in adult motor neurons, probably by controlling collagen IV emb-9 degradation, a component of the synapse basement membrane. Also involved in the organization of adult muscle morphology. Has a protease-independent function in promoting the transport from the endoplasmic reticulum to the Golgi apparatus of a variety of secretory cargos. Required for the secretion of insulin-like peptide ins-7, daf-28 and ins-18 and TGF beta-like protein daf-7. In peripheral tissues, negatively regulates insulin-mediated daf-16 translocation and thereby negatively regulates lifespan and dauer formation.
Gene References into Functions
  1. propose that GON-1 regulates a developmental switch out of an initial "pro-growth" phase during which muscle arms grow out and form synapses with motor neuron axons. PMID: 25232106
  2. ADAMTS9 and GON-1 in the ER that promotes protein transport from the ER to the Golgi. This function is GON-domain dependent but protease activity independent. PMID: 22419820
  3. GON-1 has human homologues that are in the same protease subfamily PMID: 12514189
Subcellular Location
Secreted, extracellular space, extracellular matrix, basement membrane. Endoplasmic reticulum. Golgi apparatus.
Tissue Specificity
Expressed by the gonadal distal tip cells (DTCs). Expressed in muscles, including body wall, vulval and anal depressor muscles. Expressed in motor neurons and in ASI and ASJ neurons.
Database Links

KEGG: cel:CELE_F25H8.3

STRING: 6239.F25H8.3b

UniGene: Cel.8168

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