Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HYR1 Polyclonal antibody

Uniprot No.

P40581

Target Names

HYR1

Alternative Names

HYR1 antibody; GPX3 antibody; ORP1 antibody; YIR037WGlutathione peroxidase-like peroxiredoxin HYR1 antibody; EC 1.11.1.15 antibody; Glutathione peroxidase homolog 3 antibody; GPx 3 antibody; Hydrogen peroxide resistance protein 1 antibody; Oxidant receptor peroxidase 1 antibody; Phospholipid hydroperoxide glutathione peroxidase 3 antibody; PHGPx3 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HYR1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in oxidative stress response and redox homeostasis. Functions as a sensor and transducer of hydroperoxide stress. In response to hydroperoxide stress it oxidizes (activates) the transcription activator YAP1, which is involved in transcription activation of genes of the oxidative stress response pathway. May also play a direct role in hydroperoxide scavenging, being the most active of three closely related S.cerevisiae peroxiredoxins (GPX1, GPX2, and HYR1/GPX3) with respect to peroxide and lipid hydroperoxide reduction. The three enzymes are not required for the glutaredoxin-mediated antioxidant function. In the presence of peroxides, HYR1/GPX3 is directly oxidized at Cys-36 to form a cysteine sulfenic acid (-SOH). Cys-36-SOH then forms either an intramolecular disulfide bond (Cys-36 with Cys-82) or a transient, intermolecular disulfide bond with 'Cys-598' of YAP1, which is further resolved into a YAP1 intramolecular disulfide bond ('Cys-303' with 'Cys-598'), which causes its nuclear accumulation and activation, and a reduced Cys-36 in HYR1/GPX3.

Gene References into Functions

Our data suggested that gpx3 is linked with maintenance of cellular phospholipids during Cd-induced oxidative stress. The results indicate that gpx3 might protect the phospholipids from Cd-induced oxidative damage in Saccharomyces cerevisiae. PMID: 21229313
glutathione peroxidase 3 protects GAPDH from NO stress and thereby contributes to the maintenance of homeostasis during exposure to NO stress PMID: 21229323
phospholipid hydroperoxidase and Yap1-mediated signaling activities of Gpx3 have independent functional roles PMID: 15337745
Gpx3 functions as a redox-dependent exquisite regulator of the protein repair activity of methionine sulfoxide reductase Mxr1 PMID: 16808898
Data suggested that Gpx3 protects GS from non-enzymatic proteolysis, thereby contributing to cell homeostasis when cell is exposed to oxidative stress. PMID: 17707771
Molecular mechanism of oxidative stress perception by ORP1 is reported. PMID: 17720812
Study identified 30 proteins that are related to the Gpx3-dependent oxidative stress responses and 17 proteins that are changed in a Gpx3-dependent manner regardless of oxidative stress. PMID: 18309271
The crystal structure of Hyr1 from Saccharomyces cerevisiae, is reported. PMID: 18767166
Gpx3 interacts with Dak1, alleviates dihydroxyacetone-mediated stress by upregulating Dak activity, and consequently suppresses advanced glycation endproducts formation. PMID: 19715675

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Subcellular Location

Cytoplasm. Mitochondrion intermembrane space. Peroxisome matrix.

Protein Families

Glutathione peroxidase family

Database Links

KEGG: sce:YIR037W

STRING: 4932.YIR037W

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HYR1 Antibody

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