Recombinant Saccharomyces cerevisiae Peroxiredoxin HYR1 (HYR1)

Code CSB-YP328140SVG
MSDS
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Source Yeast
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Code CSB-EP328140SVG
MSDS
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Source E.coli
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Code CSB-EP328140SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328140SVG
MSDS
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Source Baculovirus
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Code CSB-MP328140SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HYR1
Uniprot No.
Alternative Names
HYR1; GPX3; ORP1; YIR037WGlutathione peroxidase-like peroxiredoxin HYR1; EC 1.11.1.15; Glutathione peroxidase homolog 3; GPx 3; Hydrogen peroxide resistance protein 1; Oxidant receptor peroxidase 1; Phospholipid hydroperoxide glutathione peroxidase 3; PHGPx3
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-163
Target Protein Sequence
MSEFYKLAPV DKKGQPFPFD QLKGKVVLIV NVASKCGFTP QYKELEALYK RYKDEGFTII GFPCNQFGHQ EPGSDEEIAQ FCQLNYGVTF PIMKKIDVNG GNEDPVYKFL KSQKSGMLGL RGIKWNFEKF LVDKKGKVYE RYSSLTKPSS LSETIEELLK EVE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in oxidative stress response and redox homeostasis. Functions as a sensor and transducer of hydroperoxide stress. In response to hydroperoxide stress it oxidizes (activates) the transcription activator YAP1, which is involved in transcription activation of genes of the oxidative stress response pathway. May also play a direct role in hydroperoxide scavenging, being the most active of three closely related S.cerevisiae peroxiredoxins (GPX1, GPX2, and HYR1/GPX3) with respect to peroxide and lipid hydroperoxide reduction. The three enzymes are not required for the glutaredoxin-mediated antioxidant function. In the presence of peroxides, HYR1/GPX3 is directly oxidized at Cys-36 to form a cysteine sulfenic acid (-SOH). Cys-36-SOH then forms either an intramolecular disulfide bond (Cys-36 with Cys-82) or a transient, intermolecular disulfide bond with 'Cys-598' of YAP1, which is further resolved into a YAP1 intramolecular disulfide bond ('Cys-303' with 'Cys-598'), which causes its nuclear accumulation and activation, and a reduced Cys-36 in HYR1/GPX3.
Gene References into Functions
  1. Our data suggested that gpx3 is linked with maintenance of cellular phospholipids during Cd-induced oxidative stress. The results indicate that gpx3 might protect the phospholipids from Cd-induced oxidative damage in Saccharomyces cerevisiae. PMID: 21229313
  2. glutathione peroxidase 3 protects GAPDH from NO stress and thereby contributes to the maintenance of homeostasis during exposure to NO stress PMID: 21229323
  3. phospholipid hydroperoxidase and Yap1-mediated signaling activities of Gpx3 have independent functional roles PMID: 15337745
  4. Gpx3 functions as a redox-dependent exquisite regulator of the protein repair activity of methionine sulfoxide reductase Mxr1 PMID: 16808898
  5. Data suggested that Gpx3 protects GS from non-enzymatic proteolysis, thereby contributing to cell homeostasis when cell is exposed to oxidative stress. PMID: 17707771
  6. Molecular mechanism of oxidative stress perception by ORP1 is reported. PMID: 17720812
  7. Study identified 30 proteins that are related to the Gpx3-dependent oxidative stress responses and 17 proteins that are changed in a Gpx3-dependent manner regardless of oxidative stress. PMID: 18309271
  8. The crystal structure of Hyr1 from Saccharomyces cerevisiae, is reported. PMID: 18767166
  9. Gpx3 interacts with Dak1, alleviates dihydroxyacetone-mediated stress by upregulating Dak activity, and consequently suppresses advanced glycation endproducts formation. PMID: 19715675

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Subcellular Location
Cytoplasm. Mitochondrion intermembrane space. Peroxisome matrix.
Protein Families
Glutathione peroxidase family
Database Links

KEGG: sce:YIR037W

STRING: 4932.YIR037W

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