GUN4 Antibody

Datasheet

Code	CSB-PA878619XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GUN4 Polyclonal antibody

Uniprot No.

Q9LX31

Target Names

GUN4

Alternative Names

GUN4 antibody; At3g59400 antibody; F25L23_260 antibody; Tetrapyrrole-binding protein antibody; chloroplastic antibody; Genomes uncoupled 4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana GUN4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Regulates chlorophyll synthesis and plastid-to-nucleus signal transduction by binding both the product and the substrate of Mg-chelatase, an enzyme that produces magnesium-protoporphyrin IX (Mg-Proto). Activates also Mg-chelatase. Neither binds abscisic acid (ABA) nor is involved in ABA signaling.

Gene References into Functions

In conjunction with the dark repression of 5-aminolevulinic acid synthesis, GUN4 phosphorylation minimizes the flow of intermediates into the Mg branch of the tetrapyrrole metabolic pathway for chlorophyll biosynthesis. PMID: 27688621
ChlH and GUN4 use distinct mechanisms to associate with chloroplast membranes. PMID: 21467578
Data show that mutant subunits act as inhibitors of ChlH, and this inhibition is reversed upon addition of Gun4. PMID: 21110975
The physical-chemical basis for understanding GUN4 biological activity, including its role in the stimulation of Mg-chelatase activity. PMID: 15884974
GUN4 stimulates chlorophyll biosynthesis not only by activating Mg-chelatase but also by promoting interactions between ChlH and chloroplast membranes PMID: 19605356

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Subcellular Location

Plastid, chloroplast membrane; Peripheral membrane protein. Note=Might be bound to membranes by specific protein-protein interactions.

Database Links

KEGG: ath:AT3G59400

STRING: 3702.AT3G59400.1

UniGene: At.1522

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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GUN4 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background