Recombinant Arabidopsis thaliana Tetrapyrrole-binding protein, chloroplastic (GUN4)

Code CSB-YP878619DOA
MSDS
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Source Yeast
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Code CSB-EP878619DOA
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Source E.coli
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Code CSB-EP878619DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878619DOA
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Source Baculovirus
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Code CSB-MP878619DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GUN4
Uniprot No.
Alternative Names
GUN4; At3g59400; F25L23_260; Tetrapyrrole-binding protein; chloroplastic; Genomes uncoupled 4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
70-265
Target Protein Sequence
A SATTAETATI FDVLENHLVN QNFRQADEET RRLLIQISGE AAVKRGYVFF SEVKTISPED LQAIDNLWIK HSDGRFGYSV QRKIWLKVKK DFTRFFVKVE WMKLLDTEVV QYNYRAFPDE FKWELNDETP LGHLPLTNAL RGTQLLKCVL SHPAFATADD NSGETEDELN RGVAVAKEQA GVGADKRVFK TNYSF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates chlorophyll synthesis and plastid-to-nucleus signal transduction by binding both the product and the substrate of Mg-chelatase, an enzyme that produces magnesium-protoporphyrin IX (Mg-Proto). Activates also Mg-chelatase. Neither binds abscisic acid (ABA) nor is involved in ABA signaling.
Gene References into Functions
  1. In conjunction with the dark repression of 5-aminolevulinic acid synthesis, GUN4 phosphorylation minimizes the flow of intermediates into the Mg branch of the tetrapyrrole metabolic pathway for chlorophyll biosynthesis. PMID: 27688621
  2. ChlH and GUN4 use distinct mechanisms to associate with chloroplast membranes. PMID: 21467578
  3. Data show that mutant subunits act as inhibitors of ChlH, and this inhibition is reversed upon addition of Gun4. PMID: 21110975
  4. The physical-chemical basis for understanding GUN4 biological activity, including its role in the stimulation of Mg-chelatase activity. PMID: 15884974
  5. GUN4 stimulates chlorophyll biosynthesis not only by activating Mg-chelatase but also by promoting interactions between ChlH and chloroplast membranes PMID: 19605356

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Subcellular Location
Plastid, chloroplast membrane; Peripheral membrane protein. Note=Might be bound to membranes by specific protein-protein interactions.
Database Links

KEGG: ath:AT3G59400

STRING: 3702.AT3G59400.1

UniGene: At.1522

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