HAP1 Antibody

Code CSB-PA316715XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HAP1 Polyclonal antibody
Uniprot No.
Target Names
HAP1
Alternative Names
HAP1 antibody; CYP1 antibody; YLR256W antibody; L9672.1 antibody; Heme-responsive zinc finger transcription factor HAP1 antibody; CYP1 activatory protein antibody; Heme activator protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HAP1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Regulation of oxygen dependent gene expression. It modulates the expression of Iso-1 (CYP1) and Iso-2 (CYP3) cytochrome c. In response to heme, promotes transcription of genes encoding functions required for respiration, controlling oxidative damage and repression of anaerobic genes. Binds to the sequence 5'-CGGNNNTNNCGG-3'. Is non-functional in terms of iso-1 cytochrome c expression in strain S288c and its derivatives.
Gene References into Functions
  1. A simultaneous optimization of distinct intermolecular interactions determines the DNA-binding specificity of Hap1. Perturbations to individual interactions modulate Hap1 binding affinity and, very likely, its binding geometry. PMID: 15504044
  2. Hap1 binds to DNA and activates transcription of genes encoding functions required for respiration and for controlling oxidative damage. PMID: 15654089
  3. analysis of a complex mechanism of transcriptional regulation at the HAP1 promoter, involving multiple DNA elements and regulatory proteins PMID: 17706600
  4. Hap1 also plays a significant and direct role under hypoxic conditions, not as an activator, but as a repressor. PMID: 17785431
Subcellular Location
Nucleus.
Database Links

KEGG: sce:YLR256W

STRING: 4932.YLR256W

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