HDA15 Antibody

Code CSB-PA808419XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HDA15 Polyclonal antibody
Uniprot No.
Target Names
HDA15
Alternative Names
HDA15 antibody; At3g18520 antibody; MYF24_23 antibody; Histone deacetylase 15 antibody; EC 3.5.1.98 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HDA15 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (Probable). Represses chlorophyll biosynthesis and photosynthesis in the dark. Is recruited by PIF3 to the promoters of chlorophyll biosynthetic and photosynthetic genes, and represses their transcription by histone deacetylation. Involved in the repression of hypocotyl cell elongation to promote photomorphogenesis. Is recruited by HY5 to the promoters of a subset of cell wall organization and auxin signaling-related genes, and represses gene expression by decreasing the levels of histone H4 acetylation in a light-dependent manner. Promotes abscisic acid (ABA) signaling. Is recruited by MYB96 to the promoters of a subset of Rho GTPase (ROP) genes, which repress ABA signaling at the early stages of signal transduction. Represses ROP expression by removing acetyl groups of histone H3 and H4 from the cognate regions, particularly in the presence of ABA. Represses the plant response to elevated ambient temperature by directly repressing warm temperature-responsive genes.
Gene References into Functions
  1. indicate that HDA15 and PIF3 cotarget to the genes involved in chlorophyll biosynthesis and photosynthesis in the dark and repress gene expression. PMID: 23548744
  2. HDA15 shuttles in and out of the nucleus upon light exposure. PMID: 22363501
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Histone deacetylase family, HD type 2 subfamily
Tissue Specificity
Expressed in stems, leaves, flowers, siliques and mature seeds.
Database Links

KEGG: ath:AT3G18520

STRING: 3702.AT3G18520.2

UniGene: At.38541

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