Recombinant Arabidopsis thaliana Histone deacetylase 15 (HDA15), partial

Code CSB-YP808419DOA
MSDS
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Source Yeast
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Code CSB-EP808419DOA
MSDS
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Source E.coli
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Code CSB-EP808419DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP808419DOA
MSDS
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Source Baculovirus
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Code CSB-MP808419DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HDA15
Uniprot No.
Alternative Names
HDA15; At3g18520; MYF24_23; Histone deacetylase 15; EC 3.5.1.98
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (Probable). Represses chlorophyll biosynthesis and photosynthesis in the dark. Is recruited by PIF3 to the promoters of chlorophyll biosynthetic and photosynthetic genes, and represses their transcription by histone deacetylation. Involved in the repression of hypocotyl cell elongation to promote photomorphogenesis. Is recruited by HY5 to the promoters of a subset of cell wall organization and auxin signaling-related genes, and represses gene expression by decreasing the levels of histone H4 acetylation in a light-dependent manner. Promotes abscisic acid (ABA) signaling. Is recruited by MYB96 to the promoters of a subset of Rho GTPase (ROP) genes, which repress ABA signaling at the early stages of signal transduction. Represses ROP expression by removing acetyl groups of histone H3 and H4 from the cognate regions, particularly in the presence of ABA. Represses the plant response to elevated ambient temperature by directly repressing warm temperature-responsive genes.
Gene References into Functions
  1. indicate that HDA15 and PIF3 cotarget to the genes involved in chlorophyll biosynthesis and photosynthesis in the dark and repress gene expression. PMID: 23548744
  2. HDA15 shuttles in and out of the nucleus upon light exposure. PMID: 22363501
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Histone deacetylase family, HD type 2 subfamily
Tissue Specificity
Expressed in stems, leaves, flowers, siliques and mature seeds.
Database Links

KEGG: ath:AT3G18520

STRING: 3702.AT3G18520.2

UniGene: At.38541

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