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HEI10 Antibody

Rare Species
Datasheet
Code CSB-PA194047XA01DOA
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes:HEI10 Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/20000 dilution
    Predicted band size: 27.9 kDa
    Observed band size: 31 kDa
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HEI10 Polyclonal antibody
Uniprot No.
F4HRI2
Target Names
HEI10
Alternative Names
E3 ubiquitin-protein ligase CCNB1IP1 homolog (EC 2.3.2.27) (RING finger-containing protein HEI10) (RING-type E3 ubiquitin transferase HEI10) HEI10 At1g53490 F22G10.16 T3F20.19
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HEI10 protein (43-217aa)
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function
Ubiquitin E3 ligase required for class I crossover (CO) formation during meiosis.
Gene References into Functions
  1. HEI10 protein is present as numerous foci on chromosome axes during early meiotic prophase and is retained until the end of prophase at a limited number of sites corresponding to crossovers. HEI10 is structurally and functionally related to the yeast Zip3 ZMM and that it is absolutely required for class I crossover formation in Arabidopsis thaliana. [HEI10] PMID: 22844245
Subcellular Location
Nucleus. Chromosome. Note=Dynamic localization on the meiotic chromosomes. Present as numerous foci on the chromosome axes and the synaptonemal complex central element until pachytene. From pachytene to diakinesis, restricted to a limited number of sites that correspond to class I crossovers.
Tissue Specificity
Expressed mostly in flower buds and roots.
Database Links

KEGG: ath:AT1G53490

STRING: 3702.AT1G53490.1

UniGene: At.20215

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