HEL2 Antibody

Code CSB-PA445597XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HEL2 Polyclonal antibody
Uniprot No.
Target Names
HEL2
Alternative Names
HEL2 antibody; YDR266C antibody; E3 ubiquitin-protein ligase HEL2 antibody; EC 2.3.2.27 antibody; Histone E3 ligase 2 antibody; RING-type E3 ubiquitin transferase HEL2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HEL2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation.
Gene References into Functions
  1. premature arrest at ribosome stalling sequences still occurred robustly in the absence of Asc1, Hel2, and Slh1. However, these three factors were required for the ribosome quality control complex to modify the nascent chain. PMID: 28223409
  2. More detailed analyses indicated that the effects of K63R mutants were independent of the proteasome and that K63 polyubiquitination is dependent on Hel2, one of the E3 ligases. PMID: 25909477
  3. The results highlight the importance of posttranslational histone regulatory mechanisms that employ multiple E3 ubiquitin ligases such as Hel2(YDR266C)to ensure excess histone degradation and thus contribute to the maintenance of genomic stability. PMID: 22570702
Subcellular Location
Cytoplasm.
Database Links

KEGG: sce:YDR266C

STRING: 4932.YDR266C

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