Hen1 Antibody

Code CSB-PA745317XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Hen1 Polyclonal antibody
Uniprot No.
Target Names
Hen1
Alternative Names
Hen1 antibody; Pimet antibody; CG12367 antibody; Small RNA 2'-O-methyltransferase antibody; EC 2.1.1.n8 antibody; HEN1 methyltransferase homolog antibody; DmHen1 antibody; piRNA methyltransferase antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Hen1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Methyltransferase that adds a 2'-O-methyl group at the 3'-end of selected small RNAs. Mediates 2'-O-methylation of piRNAs, single-stranded siRNAs and long hairpin RNA genes (hpRNAs). Methylation protects the 3'-end of small RNAs from tailing and trimming and could constitute a recognition signal for appropriate argonaute machineries.
Gene References into Functions
  1. study found that DmHen1, the Drosophila homolog of HEN1, methylates the termini of siRNAs and piRNAsmethylates the termini of siRNAs and piRNAs. PMID: 17604629
Protein Families
Methyltransferase superfamily, HEN1 family
Database Links

KEGG: dme:Dmel_CG12367

STRING: 7227.FBpp0087092

UniGene: Dm.573

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