Recombinant Drosophila melanogaster Small RNA 2'-O-methyltransferase (Hen1)

Code CSB-YP745317DLU
MSDS
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Source Yeast
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Code CSB-EP745317DLU
MSDS
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Source E.coli
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Code CSB-EP745317DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP745317DLU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP745317DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hen1
Uniprot No.
Alternative Names
Hen1; Pimet; CG12367; Small RNA 2'-O-methyltransferase; EC 2.1.1.n8; HEN1 methyltransferase homolog; DmHen1; piRNA methyltransferase
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-391
Target Protein Sequence
MFSHKFICGS LTKMTETGIT FDPPVYEQRY CATIQILEDA RWKDQIKKVV EFGCAEMRFF QLMRRIETIE HIGLVDIDKS LLMRNLTSVN PLVSDYIRSR ASPLKVQILQ GNVADSSEEL RDTDAVIAIE LIEHVYDDVL AKIPVNIFGF MQPKLVVFST PNSDFNVIFT RFNPLLPNGF RHEDHKFEWS RDEFKNWCLG IVEKYPNYMF SLTGVGNPPK EYESVGPVSQ IAIFVRKDML EMQLVNPLVS KPNIDKESIP YKLIHTVEYP FYVDTRTEKE KLWTEVQIEL QRFKRQFESS EIEEGTYQDT CNMPIAFLLD RLEHVGATKE RIEELLLENN LTVENECVLI VSSDQESEWS DPYKFSDRSS QDDALVDQEQ EEERWDQGPE S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Methyltransferase that adds a 2'-O-methyl group at the 3'-end of selected small RNAs. Mediates 2'-O-methylation of piRNAs, single-stranded siRNAs and long hairpin RNA genes (hpRNAs). Methylation protects the 3'-end of small RNAs from tailing and trimming and could constitute a recognition signal for appropriate argonaute machineries.
Gene References into Functions
  1. study found that DmHen1, the Drosophila homolog of HEN1, methylates the termini of siRNAs and piRNAsmethylates the termini of siRNAs and piRNAs. PMID: 17604629
Protein Families
Methyltransferase superfamily, HEN1 family
Database Links

KEGG: dme:Dmel_CG12367

STRING: 7227.FBpp0087092

UniGene: Dm.573

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