Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GL2 Polyclonal antibody

Uniprot No.

P46607

Target Names

GL2

Alternative Names

GL2 antibody; ATHB10 antibody; At1g79840 antibody; F19K16.20 antibody; Homeobox-leucine zipper protein GLABRA 2 antibody; HD-ZIP protein ATHB-10 antibody; Homeobox-leucine zipper protein ATHB-10 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana GL2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor involved in the determination of epidermal cell identity (Probable). Required for correct morphological development and maturation of trichomes. Regulates the frequency of trichome initiation and determines trichome spacing. Acts as a negative factor for root hair development. Required for ectopic repression of root hair development in a subset of epidermal cells. May suppress hair formation in root epidermis by promoting differentiation into hairless epidermal cells. Directly suppresses the bHLH transcription factor genes, RHD6, RSL1, RSL2, LRL1, and LRL2, which have diverse functions in root hair development. Required for normal development of seed coat mucilage. Involved in the control of seed oil accumulation. Acts as a negative regulator of anthocyanin biosynthesis. May directly repress the expression of some component genes from the MYB-bHLH-WD40 (MBW) transcriptional activator complex. The MBW complex activates the transcription of late biosynthesis genes in the flavonoid pathway, leading to the production of anthocyanins (Probable).

Gene References into Functions

The GIR1 and GIR2 might function as adaptor proteins that associate with GL2 and participate in control of root hair formation. PMID: 28526410
These results indicate that GL2 directly suppresses basic helix-loop-helix (bHLH) genes with diverse functions in root hair development. PMID: 26486447
Deletion of the START domain from Arabidopsis Glabra2, a representative HD-Zip transcription factor, results in a complete loss-of-function phenotype. The mammalian START domain from StAR can functionally replace the HD-Zip-derived START domain. PMID: 25159688
These findings reveal distinct relationships between GL2 and single MYBs in the regulation of trichome vs root hair development, and provide new insights into the molecular mechanism of epidermal patterning. PMID: 19878461
GL2 directly regulates cell wall-related gene expression during root development. PMID: 19619157
in addition to its known function in trichome and root hair development, GLABRA2 is involved in the control of seed oil accumulation PMID: 16514561

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Subcellular Location

Nucleus.

Protein Families

HD-ZIP homeobox family, Class IV subfamily

Tissue Specificity

Expressed in individual developing trichome cells of the emerging leaf primordia. Expressed in differentiating hairless cells of root epidermis.

Database Links

KEGG: ath:AT1G79840

STRING: 3702.AT1G79840.2

UniGene: At.19986

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GL2 Antibody

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