Recombinant Arabidopsis thaliana Homeobox-leucine zipper protein GLABRA 2 (GL2), partial

Code CSB-YP341630DOA
MSDS
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Source Yeast
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Code CSB-EP341630DOA
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Source E.coli
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Code CSB-EP341630DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341630DOA
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Source Baculovirus
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Code CSB-MP341630DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GL2
Uniprot No.
Alternative Names
GL2; ATHB10; At1g79840; F19K16.20; Homeobox-leucine zipper protein GLABRA 2; HD-ZIP protein ATHB-10; Homeobox-leucine zipper protein ATHB-10
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in the determination of epidermal cell identity (Probable). Required for correct morphological development and maturation of trichomes. Regulates the frequency of trichome initiation and determines trichome spacing. Acts as a negative factor for root hair development. Required for ectopic repression of root hair development in a subset of epidermal cells. May suppress hair formation in root epidermis by promoting differentiation into hairless epidermal cells. Directly suppresses the bHLH transcription factor genes, RHD6, RSL1, RSL2, LRL1, and LRL2, which have diverse functions in root hair development. Required for normal development of seed coat mucilage. Involved in the control of seed oil accumulation. Acts as a negative regulator of anthocyanin biosynthesis. May directly repress the expression of some component genes from the MYB-bHLH-WD40 (MBW) transcriptional activator complex. The MBW complex activates the transcription of late biosynthesis genes in the flavonoid pathway, leading to the production of anthocyanins (Probable).
Gene References into Functions
  1. The GIR1 and GIR2 might function as adaptor proteins that associate with GL2 and participate in control of root hair formation. PMID: 28526410
  2. These results indicate that GL2 directly suppresses basic helix-loop-helix (bHLH) genes with diverse functions in root hair development. PMID: 26486447
  3. Deletion of the START domain from Arabidopsis Glabra2, a representative HD-Zip transcription factor, results in a complete loss-of-function phenotype. The mammalian START domain from StAR can functionally replace the HD-Zip-derived START domain. PMID: 25159688
  4. These findings reveal distinct relationships between GL2 and single MYBs in the regulation of trichome vs root hair development, and provide new insights into the molecular mechanism of epidermal patterning. PMID: 19878461
  5. GL2 directly regulates cell wall-related gene expression during root development. PMID: 19619157
  6. in addition to its known function in trichome and root hair development, GLABRA2 is involved in the control of seed oil accumulation PMID: 16514561

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Subcellular Location
Nucleus.
Protein Families
HD-ZIP homeobox family, Class IV subfamily
Tissue Specificity
Expressed in individual developing trichome cells of the emerging leaf primordia. Expressed in differentiating hairless cells of root epidermis.
Database Links

KEGG: ath:AT1G79840

STRING: 3702.AT1G79840.2

UniGene: At.19986

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