Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HKT1 Polyclonal antibody

Uniprot No.

Q84TI7

Target Names

HKT1

Alternative Names

HKT1 antibody; At4g10310 antibody; T9A4.5Sodium transporter HKT1 antibody; AtHKT1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana HKT1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Sodium transporter protein, which plays a central role in plant tolerance to salt. Upon prolongated exposure to high concentrations, Na(+) translocates from the roots to the transpiring leaves where it can increase to toxic level. Involved in Na(+) recirculation from shoots to roots, probably by mediating Na(+) loading into the phloem sap in shoots and unloading in roots, thereby removing large amounts of Na(+) from the shoot. Does not transport K(+) but regulates K(+) nutrient status via its ability to facilitate Na(+) homeostasis. Probably not involved in root uptake of Na(+).

Gene References into Functions

The amino-acid substitutionof Asp, replacing Asn in HKT1 altered cation selectivity and uptake dynamics and lead to plant salt tolerance. PMID: 27208305
broad range of metabolites and elements in shoots and roots of different AtHKT1;1 genotypes PMID: 23132143
ABI4 binds two highly related sites within the HKT1;1 promoter.ABI4 downregulates expression of the sodium transporter HKT1;1 in Arabidopsis roots. PMID: 23240817
AtHKT1;1 mediates nernstian sodium channel transport properties in Arabidopsis root stelar cells. PMID: 21931830
Cytokinin acts through ARR1 and ARR12 to regulate AtHKT1;1 expression. PMID: 21105923
Cis-regulatory expression level polymorphisms at the AtHKT1;1 locus, encoding a known sodium (Na(+)) transporter, was identified as being a major factor controlling natural variation in leaf Na(+) accumulation capacity in the A. thaliana population. PMID: 21085628
Overexpress AtHKT1 in the cortex and epidermis upregulated the native AtHKT1. PMID: 20838445
Studies demonstrated that a class of xylem-parenchyma-expressed Na(+)-permeable plant HKT transporters represent a primary mechanism mediating salt tolerance and Na(+) exclusion from leaves. PMID: 19783197
controls Na(+) homeostasis and through this function regulates K(+) nutrient status PMID: 15347798
AtHKT1 selectively unloads sodium directly from xylem vessels to xylem parenchyma cells. PMID: 16359386
SOS3 and AtHKT1 are major determinants of salinity resistance such that sos3 more strongly causes Na+ overaccumulation in roots, whereas athkt1 causes an increase in Na+ levels in the xylem sap and shoots and a concomitant Na+ reduction in roots. PMID: 16540484
Using DNA microarray-based genotyping, identified AtHKT1 as the casual locus driving the natural variation in shoot Na(+) accumulation we observed in Ts-1 and Tsu-1 PMID: 17140289
AtHKT1;1 contributes to the control of both root accumulation of Na(+) and retrieval of Na(+) from the xylem, but is not involved in root influx or recirculation in the phloem. PMID: 17324235
Results demonstrate that tissue-specific regulation of HKT1 is critical for managing Na(+) homeostasis in salt-stressed plants, as well as underscore the breadth and sophistication of plant-microbe interactions PMID: 18624638
Plants constitutively expressing HKT1;1 driven by the cauliflower mosaic virus 35S promoter accumulated high shoot Na+ and grew poorly. PMID: 19584143

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

TrkH potassium transport family, HKT (TC 2.A.38.3) subfamily

Tissue Specificity

Highly expressed in roots. Expressed in flowers, leaves and stems. Expressed in the vascular tissues of every organs. In roots, leaves and flower peduncles, it is only expressed in the phloem tissues. Not expressed in root peripheral cells.

Database Links

KEGG: ath:AT4G10310

STRING: 3702.AT4G10310.1

UniGene: At.2986

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HKT1 Antibody

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