Product Details

Purity

>85% (SDS-PAGE)

Target Names

HKT1

Uniprot No.

Q84TI7

Alternative Names

HKT1; At4g10310; T9A4.5Sodium transporter HKT1; AtHKT1

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Sodium transporter protein, which plays a central role in plant tolerance to salt. Upon prolongated exposure to high concentrations, Na(+) translocates from the roots to the transpiring leaves where it can increase to toxic level. Involved in Na(+) recirculation from shoots to roots, probably by mediating Na(+) loading into the phloem sap in shoots and unloading in roots, thereby removing large amounts of Na(+) from the shoot. Does not transport K(+) but regulates K(+) nutrient status via its ability to facilitate Na(+) homeostasis. Probably not involved in root uptake of Na(+).

Gene References into Functions

The amino-acid substitutionof Asp, replacing Asn in HKT1 altered cation selectivity and uptake dynamics and lead to plant salt tolerance. PMID: 27208305
broad range of metabolites and elements in shoots and roots of different AtHKT1;1 genotypes PMID: 23132143
ABI4 binds two highly related sites within the HKT1;1 promoter.ABI4 downregulates expression of the sodium transporter HKT1;1 in Arabidopsis roots. PMID: 23240817
AtHKT1;1 mediates nernstian sodium channel transport properties in Arabidopsis root stelar cells. PMID: 21931830
Cytokinin acts through ARR1 and ARR12 to regulate AtHKT1;1 expression. PMID: 21105923
Cis-regulatory expression level polymorphisms at the AtHKT1;1 locus, encoding a known sodium (Na(+)) transporter, was identified as being a major factor controlling natural variation in leaf Na(+) accumulation capacity in the A. thaliana population. PMID: 21085628
Overexpress AtHKT1 in the cortex and epidermis upregulated the native AtHKT1. PMID: 20838445
Studies demonstrated that a class of xylem-parenchyma-expressed Na(+)-permeable plant HKT transporters represent a primary mechanism mediating salt tolerance and Na(+) exclusion from leaves. PMID: 19783197
controls Na(+) homeostasis and through this function regulates K(+) nutrient status PMID: 15347798
AtHKT1 selectively unloads sodium directly from xylem vessels to xylem parenchyma cells. PMID: 16359386
SOS3 and AtHKT1 are major determinants of salinity resistance such that sos3 more strongly causes Na+ overaccumulation in roots, whereas athkt1 causes an increase in Na+ levels in the xylem sap and shoots and a concomitant Na+ reduction in roots. PMID: 16540484
Using DNA microarray-based genotyping, identified AtHKT1 as the casual locus driving the natural variation in shoot Na(+) accumulation we observed in Ts-1 and Tsu-1 PMID: 17140289
AtHKT1;1 contributes to the control of both root accumulation of Na(+) and retrieval of Na(+) from the xylem, but is not involved in root influx or recirculation in the phloem. PMID: 17324235
Results demonstrate that tissue-specific regulation of HKT1 is critical for managing Na(+) homeostasis in salt-stressed plants, as well as underscore the breadth and sophistication of plant-microbe interactions PMID: 18624638
Plants constitutively expressing HKT1;1 driven by the cauliflower mosaic virus 35S promoter accumulated high shoot Na+ and grew poorly. PMID: 19584143

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

TrkH potassium transport family, HKT (TC 2.A.38.3) subfamily

Tissue Specificity

Highly expressed in roots. Expressed in flowers, leaves and stems. Expressed in the vascular tissues of every organs. In roots, leaves and flower peduncles, it is only expressed in the phloem tissues. Not expressed in root peripheral cells.

Database Links

KEGG: ath:AT4G10310

STRING: 3702.AT4G10310.1

UniGene: At.2986

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Recombinant Arabidopsis thaliana Sodium transporter HKT1 (HKT1), partial

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