Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HOS1 Polyclonal antibody

Uniprot No.

Q84JU6

Target Names

HOS1

Alternative Names

HOS1 antibody; At2g39810 antibody; T5I7.11E3 ubiquitin-protein ligase HOS1 antibody; EC 2.3.2.27 antibody; Protein HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 antibody; RING finger protein HOS1 antibody; RING-type E3 ubiquitin transferase HOS1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana HOS1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of the transcription factor ICE1. Acts as a negative regulator of cold signaling pathways. Probably involved in recruiting the NUP107-160 subcomplex of the nuclear pore complex to chromatin (Probable). Controls flowering time in response to ambient temperatures (16 and 23 degrees Celsius) and intermittent cold, probably via the regulation of FT and TSF levels.

Gene References into Functions

While HOS1 is known to act as an E3 ubiquitin ligase or a chromatin remodeling factor, data illustrate a novel role of HOS1: it acts as a component of phyB-mediated light signaling in hypocotyl photomorphogenesis. PMID: 28426369
HOS1 is intimately linked with hypocotyl growth, in which HOS1 inhibits the transcriptional activation activity of PIF4 PMID: 27890635
Red light-mediated degradation of CO protein by HOS1 ligase regulates photoperiodic flowering in Arabidopsis.HOS1 and PHYB regulate flowering time additively. PMID: 26373454
HOS1 regulates miR168a/b and AGO1 levels in Arabidopsis by maintaining proper transcription of MIR168b. PMID: 25619693
HOS1 regulates FLC transcription via chromatin remodeling. PMID: 24390058
HOS1 is required for circadian periodicity through the promotion of nucleo-cytoplasmic mRNA export. PMID: 24254125
HOS1 acts as a chromatin remodeling factor for FLC regulation under short-term cold stress. PMID: 24220632
HOS1 acts as a common regulator in the signaling pathways that control flowering time in response to low ambient temperature. PMID: 22960247
the HOS1-CO module contributes to the fine-tuning of photoperiodic flowering under short term temperature fluctuations, which often occur during local weather disturbances. PMID: 23135282
HOS1-L transcripts were spatio-temporally more abundant than those of HOS1-S and the expression was affected by photoperiod. PMID: 23010172
HOS1 is required to modulate precisely the timing of CO accumulation and that this regulation is essential to maintain low levels of FT during the first part of the day and, subsequently, a correct photoperiodic response in Arabidopsis. PMID: 22408073
cold stress responses in Arabidopsis are attenuated by a ubiquitination/proteasome pathway in which HOS1 mediates the degradation of the ICE1 protein PMID: 16702557

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Subcellular Location

Nucleus. Cytoplasm.

Tissue Specificity

Ubiquitously expressed with higher levels in leaf vasculature, roots and root tips.

Database Links

KEGG: ath:AT2G39810

STRING: 3702.AT2G39810.1

UniGene: At.37128

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HOS1 Antibody

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