Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HSP90-2 Polyclonal antibody

Uniprot No.

P55737

Target Names

HSP90-2

Alternative Names

HSP90-2 antibody; ERD8 antibody; HSP81-2 antibody; LRA2 antibody; MUSE12 antibody; At5g56030 antibody; MDA7.7Heat shock protein 90-2 antibody; AtHSP90.2 antibody; AtHsp90-2 antibody; Heat shock protein 81-2 antibody; Hsp81-2 antibody; Protein EARLY-RESPONSIVE TO DEHYDRATION 8 antibody; Protein LOSS OF RECOGNITION OF AVRRPM1 2 antibody; Protein MUTANT SNC1-ENHANCING 12 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana HSP90-2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Molecular chaperone. Involved in RPM1-mediated resistance. Component of the RPM1/RAR1/SGT1 complex. May stabilize RPM1 and protect it from SGT1-mediated degradation. Associates with RAR1 which may function as co-chaperone. Possesses ATPase activity. In the absence of heat shock, negatively regulates heat-inducible genes by actively suppressing heat shock transcription factor A1D (HSFA1D) function. Involved in the induction of heat shock transcription factor A2 (HSFA2) expression in response to oxidative stress. Required for stomatal closure and modulates transcriptional and physiological responses to abscisic acid (ABA). Regulates RPP4-mediated temperature-dependent cell death and defense responses. May assist SGT1B in the formation of SCF E3 ubiquitin ligase complexes that target the immune receptors SNC1, RPS2 and RPS4 for degradation, to regulate receptor levels and avoid autoimmunity.

Gene References into Functions

The regulation of AtHSP90-1 and AtHSP90-3 proteins during seed development in A. thaliana. [HSP90-3] PMID: 15582930
HSP90.2 is involved in the heat shock response in A. thaliana. It interacts with AtHsfA7a and AtHsfB1 but only weakly interacts or does not interact with AtHsfA4c. PMID: 17965410
Nuclear magnetic resonance spectroscopy and mutational analysis of HSP90 revealed the nature of its binding with the CS domain of SGT1 which is required for innate immunity in plants and animals. [HSP90] PMID: 18833289

Subcellular Location

Cytoplasm.

Protein Families

Heat shock protein 90 family

Tissue Specificity

Present in all tissues. Most abundantly expressed in roots followed by floral bud clusters, flowers and young fruits.

Database Links

KEGG: ath:AT5G56030

UniGene: At.25243

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HSP90-2 Antibody

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