Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) IAA3 Polyclonal antibody

Uniprot No.

Q38822

Target Names

IAA3

Alternative Names

IAA3 antibody; SHY2 antibody; At1g04240 antibody; F19P19.32 antibody; Auxin-responsive protein IAA3 antibody; Indoleacetic acid-induced protein 3 antibody; Short hypocotyl antibody; Suppressor of HY2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana IAA3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Plays a central role in auxin regulation of root growth, in gravitropism, and in lateral root formation. Regulated by an auxin-induced protein turnover. Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. When activated by cytokinin, restricts the expression of the PIN genes to the vascular transition zone. Induction of SHY2 in the vascular transition zone restricts BRX expression to down-regulate PIN3 and thus limit meristem growth, but proper SHY2 expression requires BRX. Involved in meristem growth and in determining its size. May participate in strigolactone signaling to regulate meristem size and lateral root formation.

Gene References into Functions

SHY2 is a pivotal negative regulator of root growth, which has been demonstrated to efficiently repress transcription of major auxin transport facilitators of the PIN-FORMED (PIN) gene family, thereby restricting polar auxin transport to the root tip and in consequence auxin-driven primary root growth. Taken together, our results disclose the central low-energy activated SnRK1-C/S1-bZIP signalling module as gateway to PMID: 28158182
DA1-related protein 2 (DAR2) acts downstream of cytokinin and SHY2 but upstream of PLT1/2 to affect root meristem size. PMID: 23518585
Strigolactone signaling involves SHY2 protein activity in roots. PMID: 23425316
The shy2 mutations strongly elevate endogenous indole-3-acetic acid level in the roots. PMID: 22527388
Data suggest a model in which the regulatory interactions favor BRX expression in the early proximal meristem and SHY2 prevails because of supplementary cytokinin induction in the later distal meristem. PMID: 21149702

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Subcellular Location

Nucleus.

Protein Families

Aux/IAA family

Tissue Specificity

Highly expressed in stems and flowers. Expressed in hypocotyls, cotyledons and leaves, but barely detected in roots. Expressed in root tips. In the root meristem, specifically detected at the vascular tissue transition zone.

Database Links

KEGG: ath:AT1G04240

STRING: 3702.AT1G04240.1

UniGene: At.20801

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IAA3 Antibody

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