SCRM Antibody

Code CSB-PA881796XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SCRM Polyclonal antibody
Uniprot No.
Target Names
SCRM
Alternative Names
SCRM antibody; BHLH116 antibody; EN45 antibody; ICE1 antibody; At3g26744 antibody; MDJ14.1 antibody; MLJ15.14Transcription factor ICE1 antibody; Basic helix-loop-helix protein 116 antibody; AtbHLH116 antibody; bHLH 116 antibody; Inducer of CBF expression 1 antibody; Transcription factor EN 45 antibody; Transcription factor SCREAM antibody; bHLH transcription factor bHLH116 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SCRM protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. Functions as a dimer with SPCH during stomatal initiation.
Gene References into Functions
  1. MPK3 and MPK6 can phosphorylate ICE1, a basic-helix-loop-helix transcription factor that regulates the expression of CBF genes PMID: 29056551
  2. unified ICE-CBF pathway provides transcriptional feedback control of freezing tolerance during cold acclimation PMID: 26311645
  3. Seedling growth was severely reduced in a T-DNA insertion mutant of ICE1, ice1-2, when grown on 1/2 MS medium lacking sugars, but was restored to wild-type (WT) levels by supplementation with 56 mM glucose. PMID: 26048037
  4. ICE2 gene has originated from a duplication event about 17.9MYA followed by sub- and neofunctionalization of the ancestral ICE1 gene. PMID: 25443829
  5. uncover the unexpected roles of OST1 in modulating C-repeat-binding factor-dependent cold signaling in Arabidopsis PMID: 25669882
  6. propose that the ZHOUPI/ICE1 complex might have ancient origins, acquiring novel megagametophyte-specific functions in heterosporous land plants that were conserved in the angiosperm endosperm PMID: 24553285
  7. Jasmonate functions as a critical upstream signal of the ICE-CBF/DREB1 pathway to positively regulate Arabidopsis freezing tolerance. PMID: 23933884
  8. The serine 403 to alanine substitution increases the transactivation activity of ICE1 and the freezing tolerance of Arabidopsis. PMID: 21447070
  9. The inducer of CBF expression1 (ICE1) protein that is involved in transcriptional control of cold responses is found to bind to a MYC element in this BAP1 promoter and is required for the cooling induction of BAP1. PMID: 21098676
  10. Regulon genes repressed by siz1 did not affect expression of ICE1, which encodes a MYC transcription factor. PMID: 17416732

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Subcellular Location
Nucleus.
Tissue Specificity
Widely expressed in the whole plant with high expression in leaves and stem. Broad expression within stomatal cell lineages of leaf epidermis.
Database Links

KEGG: ath:AT3G26744

STRING: 3702.AT3G26744.1

UniGene: At.25302

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