Recombinant Arabidopsis thaliana Transcription factor ICE1 (SCRM)

Code CSB-YP881796DOA
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Source Yeast
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Code CSB-EP881796DOA
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Source E.coli
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Code CSB-EP881796DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881796DOA
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Source Baculovirus
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Code CSB-MP881796DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SCRM
Uniprot No.
Alternative Names
SCRM; BHLH116; EN45; ICE1; At3g26744; MDJ14.1; MLJ15.14Transcription factor ICE1; Basic helix-loop-helix protein 116; AtbHLH116; bHLH 116; Inducer of CBF expression 1; Transcription factor EN 45; Transcription factor SCREAM; bHLH transcription factor bHLH116
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-494
Target Protein Sequence
MGLDGNNGGG VWLNGGGGER EENEEGSWGR NQEDGSSQFK PMLEGDWFSS NQPHPQDLQM LQNQPDFRYF GGFPFNPNDN LLLQHSIDSS SSCSPSQAFS LDPSQQNQFL STNNNKGCLL NVPSSANPFD NAFEFGSESG FLNQIHAPIS MGFGSLTQLG NRDLSSVPDF LSARSLLAPE SNNNNTMLCG GFTAPLELEG FGSPANGGFV GNRAKVLKPL EVLASSGAQP TLFQKRAAMR QSSGSKMGNS ESSGMRRFSD DGDMDETGIE VSGLNYESDE INESGKAAES VQIGGGGKGK KKGMPAKNLM AERRRRKKLN DRLYMLRSVV PKISKMDRAS ILGDAIDYLK ELLQRINDLH NELESTPPGS LPPTSSSFHP LTPTPQTLSC RVKEELCPSS LPSPKGQQAR VEVRLREGRA VNIHMFCGRR PGLLLATMKA LDNLGLDVQQ AVISCFNGFA LDVFRAEQCQ EGQEILPDQI KAVLFDTAGY AGMI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. Functions as a dimer with SPCH during stomatal initiation.
Gene References into Functions
  1. MPK3 and MPK6 can phosphorylate ICE1, a basic-helix-loop-helix transcription factor that regulates the expression of CBF genes PMID: 29056551
  2. unified ICE-CBF pathway provides transcriptional feedback control of freezing tolerance during cold acclimation PMID: 26311645
  3. Seedling growth was severely reduced in a T-DNA insertion mutant of ICE1, ice1-2, when grown on 1/2 MS medium lacking sugars, but was restored to wild-type (WT) levels by supplementation with 56 mM glucose. PMID: 26048037
  4. ICE2 gene has originated from a duplication event about 17.9MYA followed by sub- and neofunctionalization of the ancestral ICE1 gene. PMID: 25443829
  5. uncover the unexpected roles of OST1 in modulating C-repeat-binding factor-dependent cold signaling in Arabidopsis PMID: 25669882
  6. propose that the ZHOUPI/ICE1 complex might have ancient origins, acquiring novel megagametophyte-specific functions in heterosporous land plants that were conserved in the angiosperm endosperm PMID: 24553285
  7. Jasmonate functions as a critical upstream signal of the ICE-CBF/DREB1 pathway to positively regulate Arabidopsis freezing tolerance. PMID: 23933884
  8. The serine 403 to alanine substitution increases the transactivation activity of ICE1 and the freezing tolerance of Arabidopsis. PMID: 21447070
  9. The inducer of CBF expression1 (ICE1) protein that is involved in transcriptional control of cold responses is found to bind to a MYC element in this BAP1 promoter and is required for the cooling induction of BAP1. PMID: 21098676
  10. Regulon genes repressed by siz1 did not affect expression of ICE1, which encodes a MYC transcription factor. PMID: 17416732

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Subcellular Location
Nucleus.
Tissue Specificity
Widely expressed in the whole plant with high expression in leaves and stem. Broad expression within stomatal cell lineages of leaf epidermis.
Database Links

KEGG: ath:AT3G26744

STRING: 3702.AT3G26744.1

UniGene: At.25302

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