EIF4E1 Antibody

Code CSB-PA517801XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EIF4E1 Polyclonal antibody
Uniprot No.
Target Names
EIF4E1
Alternative Names
EIF4E1 antibody; CUM1 antibody; At4g18040 antibody; F15J5.10 antibody; Eukaryotic translation initiation factor 4E-1 antibody; eIF-4E-1 antibody; eIF4E-1 antibody; Protein cucumovirus multiplication 1 antibody; eIF-4F 25 kDa subunit antibody; eIF-4F p26 subunit antibody; mRNA cap-binding protein antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EIF4E1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Gene References into Functions
  1. Results indicate that nCBP is a novel member of the eIF4E family recessive resistance genes whose loss impairs viral cell-to-cell movement by inhibiting the efficient accumulation of triple-gene-block protein 2 and triple-gene-block protein 3. PMID: 28059075
  2. analysis of eIF4E isoforms from Arabidopsis thaliana that have distinct features of cap analogs binding PMID: 25446076
  3. These results indicate that selective translation mediated by eIF(iso)4E is relevant for Arabidopsis root development under normal growth conditions. PMID: 22363683
  4. Turnip mosaic virus (TuMV), another member of the genus Potyvirus, multiplied in mutant plants lacking eIF4E but not in mutant plants lacking eIF(iso)4E PMID: 15710407
Protein Families
Eukaryotic initiation factor 4E family
Tissue Specificity
Expressed in all tissues except in the cells of the specialization zone of the roots.
Database Links

KEGG: ath:AT4G18040

STRING: 3702.AT4G18040.1

UniGene: At.40

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