Recombinant Arabidopsis thaliana Eukaryotic translation initiation factor 4E-1 (EIF4E1)

Code CSB-YP517801DOA
MSDS
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Source Yeast
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Code CSB-EP517801DOA
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Source E.coli
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Code CSB-EP517801DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP517801DOA
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Source Baculovirus
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Code CSB-MP517801DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EIF4E1
Uniprot No.
Alternative Names
EIF4E1; CUM1; At4g18040; F15J5.10; Eukaryotic translation initiation factor 4E-1; eIF-4E-1; eIF4E-1; Protein cucumovirus multiplication 1; eIF-4F 25 kDa subunit; eIF-4F p26 subunit; mRNA cap-binding protein
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-235
Target Protein Sequence
MAVEDTPKSV VTEEAKPNSI ENPIDRYHEE GDDAEEGEIA GGEGDGNVDE SSKSGVPESH PLEHSWTFWF DNPAVKSKQT SWGSSLRPVF TFSTVEEFWS LYNNMKHPSK LAHGADFYCF KHIIEPKWED PICANGGKWT MTFPKEKSDK SWLYTLLALI GEQFDHGDEI CGAVVNIRGK QERISIWTKN ASNEAAQVSI GKQWKEFLDY NNSIGFIIHE DAKKLDRNAK NAYTA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Gene References into Functions
  1. Results indicate that nCBP is a novel member of the eIF4E family recessive resistance genes whose loss impairs viral cell-to-cell movement by inhibiting the efficient accumulation of triple-gene-block protein 2 and triple-gene-block protein 3. PMID: 28059075
  2. analysis of eIF4E isoforms from Arabidopsis thaliana that have distinct features of cap analogs binding PMID: 25446076
  3. These results indicate that selective translation mediated by eIF(iso)4E is relevant for Arabidopsis root development under normal growth conditions. PMID: 22363683
  4. Turnip mosaic virus (TuMV), another member of the genus Potyvirus, multiplied in mutant plants lacking eIF4E but not in mutant plants lacking eIF(iso)4E PMID: 15710407
Protein Families
Eukaryotic initiation factor 4E family
Tissue Specificity
Expressed in all tissues except in the cells of the specialization zone of the roots.
Database Links

KEGG: ath:AT4G18040

STRING: 3702.AT4G18040.1

UniGene: At.40

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