Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIF4632 Polyclonal antibody

Uniprot No.

P39936

Target Names

TIF4632

Alternative Names

TIF4632 antibody; YGL049C antibody; Eukaryotic initiation factor 4F subunit p130 antibody; eIF-4F p130 antibody; eIF4F p130 antibody; eIF4G2 antibody; mRNA cap-binding protein complex subunit p130 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIF4632 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the eIF4F complex, which interacts with the mRNA cap structure and serves as an initial point of assembly for the translation apparatus. Stimulates translation by interaction with polyadenylate-binding protein PAB1, bringing the 5'- and 3'-ends of the mRNA in proximity. The formation of this circular mRNP structure appears to be critical for the synergistic effects of the cap and the poly(A) tail in facilitating translation initiation, recycling of ribosomes, and mRNA stability. TIF4632 is probably essential when TIF4631 is missing.

Gene References into Functions

depleting eukaryotic translation initiation factor 4G (eIF4G), the scaffold subunit of eukaryotic translation initiation factor 4F (eIF4F), preferentially impacts short mRNAs with strong closed-loop potential and unstructured 5' UTRs, exactly the opposite features associated with hyperdependence on the eIF4B/helicases. PMID: 27601676
These results reveal and characterize an unexpected interdependence between the two RNA helicases and eIF4G, and suggest that Ded1p is an integral part of eIF4F, the complex comprising eIF4G, eIF4A, and eIF4E. PMID: 27494274
Data show that that deletion of eIF4G1, but not eIF4G2, impairs growth and global translation initiation rates. PMID: 20161741
involvement of yeast eIF4G in pre-mRNA metabolism; Tif4631p and Tif4632p interact efficiently with U1 snRNA; Tif4631p and Tif4632p associate with protein components of the splicing machinery, namely Snu71p and Prp11p. PMID: 19838078
structure of the complex formed by yeast eIF4G's middle domain and full-length eIF4A at 2.6-A resolution PMID: 18606994

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Subcellular Location

Cytoplasm.

Protein Families

Eukaryotic initiation factor 4G family

Database Links

KEGG: sce:YGL049C

STRING: 4932.YGL049C

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TIF4632 Antibody

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