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Recombinant Saccharomyces cerevisiae Eukaryotic initiation factor 4F subunit p130 (TIF4632), partial

Recombinant Saccharomyces cerevisiae Eukaryotic initiation factor 4F subunit p130 (TIF4632), partial

Code	CSB-YP340179SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP340179SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP340179SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP340179SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP340179SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

TIF4632

Uniprot No.

P39936

Alternative Names

TIF4632; YGL049C; Eukaryotic initiation factor 4F subunit p130; eIF-4F p130; eIF4F p130; eIF4G2; mRNA cap-binding protein complex subunit p130

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Component of the eIF4F complex, which interacts with the mRNA cap structure and serves as an initial point of assembly for the translation apparatus. Stimulates translation by interaction with polyadenylate-binding protein PAB1, bringing the 5'- and 3'-ends of the mRNA in proximity. The formation of this circular mRNP structure appears to be critical for the synergistic effects of the cap and the poly(A) tail in facilitating translation initiation, recycling of ribosomes, and mRNA stability. TIF4632 is probably essential when TIF4631 is missing.

Gene References into Functions

depleting eukaryotic translation initiation factor 4G (eIF4G), the scaffold subunit of eukaryotic translation initiation factor 4F (eIF4F), preferentially impacts short mRNAs with strong closed-loop potential and unstructured 5' UTRs, exactly the opposite features associated with hyperdependence on the eIF4B/helicases. PMID: 27601676
These results reveal and characterize an unexpected interdependence between the two RNA helicases and eIF4G, and suggest that Ded1p is an integral part of eIF4F, the complex comprising eIF4G, eIF4A, and eIF4E. PMID: 27494274
Data show that that deletion of eIF4G1, but not eIF4G2, impairs growth and global translation initiation rates. PMID: 20161741
involvement of yeast eIF4G in pre-mRNA metabolism; Tif4631p and Tif4632p interact efficiently with U1 snRNA; Tif4631p and Tif4632p associate with protein components of the splicing machinery, namely Snu71p and Prp11p. PMID: 19838078
structure of the complex formed by yeast eIF4G's middle domain and full-length eIF4A at 2.6-A resolution PMID: 18606994

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Subcellular Location

Cytoplasm.

Protein Families

Eukaryotic initiation factor 4G family

Database Links

KEGG: sce:YGL049C

STRING: 4932.YGL049C

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