Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KAP95 Polyclonal antibody

Uniprot No.

Q06142

Target Names

KAP95

Alternative Names

Importin subunit beta-1 (Importin-95) (Karyopherin subunit beta-1) (Karyopherin-95) KAP95 YLR347C L8300.15

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KAP95 protein (611-861aa)

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, GTP-Ran binds to importin beta and the three components separate, leading to release of the cargo. Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of histones H2A and H2B. Mediates the nuclear import of transcription factor GCN4.

Gene References into Functions

Data suggest that, in nuclear exosomes, karyopherins are bound to the Rrp6 complex; Srp1, Kap95, and Sxm1 appear to be the most important karyopherins for nuclear import of Rrp6. (Rrp6 = exosome nuclease subunit RRP6; Srp1 = karyopherin alpha kap60; Kap95 = karyopherin beta Kap95; Sxm1 = karyopherin Kap108) PMID: 28539363
The Kap95 is able to mediate efficient Mig2-Kap95 interaction in the absence of the importin-alpha. PMID: 23131016
Hxk2 as a new cargo for the alpha/beta-importin pathway of S. cerevisiae. PMID: 22157003
Kap95 is likely the primary karyopherin responsible for Replication protein A nuclear import in yeast. PMID: 21332387
The authors identified karyopherin Kap95 as the transport factor responsible for the nuclear import of Slt2 and Rlm1. PMID: 19930464
identification of a key interaction site where the RanGTP switch I loop binds to the carboxy-terminal arch of Kap95p PMID: 15864302
There is a correlation between the binding affinity of an NLS cargo for the NLS receptor, importin alpha, and the import rate for this cargo. PMID: 16785238
Kap95 serves as a key regulator of nuclear pore complex assembly into intact nuclear envelopes. PMID: 17182855
Data show the crystal structure of the Kap95p-RanGDP complex shows that Kap95p induces the switch I and II regions of RanGDP to adopt a conformation that resembles that of the GTP-bound form. PMID: 18708071
Pct1 interacts with Kap60 and Kap95 with this tripartite complex transiting the nuclear pore. PMID: 19141610

Hide All

Subcellular Location

Cytoplasm. Nucleus. Nucleus, nuclear pore complex.

Protein Families

Importin beta family, Importin beta-1 subfamily

Database Links

KEGG: sce:YLR347C

STRING: 4932.YLR347C

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Code	CSB-PA012490XA01SVG
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes:KAP95 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 34.6 kDa Observed band size: 34 kDa
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KAP95 Antibody

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